|本期目录/Table of Contents|

[1]李丹,梁锡宏,李政威,等.一株同步硝化-反硝化菌的絮凝特性[J].生物加工过程,2019,17(01):104-109.[doi:10.3969/j.issn.1672-3678.2019.01.014]
 LI Dan,LIANG Xihong,LI Zhengwei,et al.Aggregation characteristics of a nitrifying-denitrifying bacterium[J].Chinese Journal of Bioprocess Engineering,2019,17(01):104-109.[doi:10.3969/j.issn.1672-3678.2019.01.014]
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一株同步硝化-反硝化菌的絮凝特性()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
17
期数:
2019年01期
页码:
104-109
栏目:
出版日期:
2019-01-30

文章信息/Info

Title:
Aggregation characteristics of a nitrifying-denitrifying bacterium
文章编号:
1672-3678(2019)01-0104-06
作者:
李丹梁锡宏李政威金垚吴重德周荣清
四川大学 轻纺与食品学院 皮革化学与工程教育部重点实验室,四川 成都 610065
Author(s):
LI DanLIANG XihongLI ZhengweiJIN YaoWU ChongdeZHOU Rongqing
Key Laboratory of Leather Chemistry and Engineering of the Ministry of Education,College of Light Industry, Textile & Food Engineering,Sichuan University,Chengdu 610065,China
关键词:
同步硝化-反硝化菌 自聚集 胞外聚合物 絮凝 脱氮细菌
分类号:
X172
DOI:
10.3969/j.issn.1672-3678.2019.01.014
文献标志码:
A
摘要:
研究了1株克雷伯氏菌(Klebsiella sp.TN-10)的硝化反硝化性能,结果表明,该菌能高效降解铵态氮(NH+4)、硝态氮(NO-3)和亚硝态氮(NO-2)。在初始NH+4质量浓度为77.93 mg/L的培养基中,Klebsiella sp.TN-10能够在20 h内降解93.3%的NH+4,其硝化速率为3.02 mg/(L·h); 在初始NO-3和NO-2质量浓度分别为64.03和87.68 mg/L的培养基中,TN-10能够在40 h内降解95.44%的NO-3和93.44%的NO-2。测定了该菌株在不同生长周期的自聚集能力和聚集动力学,结果表明,该菌在衰亡期呈现更好的自聚集性。对菌株TN-10的胞外聚合物(EPS)组成进行了研究,结果表明,其蛋白质含量为23.84 mg(以每克干细胞质量计),多糖含量为18.64 mg(以每克干细胞质量计)。红外图谱表明,EPS主要成分为蛋白质和多糖。测定EPS的圆二图谱,对EPS中蛋白质二级结构进行深入分析,结果表明α-螺旋占主要比例,为55.08%。本研究对深入认识TN-10的特点具有重要意义,也可为硝化反硝化菌胞外聚合物研究提供重要参考。

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备注/Memo

备注/Memo:
收稿日期:2018-09-01修回日期:2018-09-08
基金项目:国家重点研发计划(2017YFB0308500); 四川大学跨学科专业——贯通式人才培养建设平台项目(SCUKG017); 大学生创新创业计划(C2018101384)
作者简介:李丹(1993—),女,四川简阳人,硕士研究生,研究方向:环境微生物; 吴重德(联系人),副教授,E-mail:cdwu@scu.edu.cn
引文格式:李丹,梁锡宏,李政威,等.一株同步硝化-反硝化菌的絮凝特性[J].生物加工过程,2019,17(1):104-109.
LI Dan,LIANG Xihong,LI Zhengwei,et al.Aggregation characteristics of a nitrifying-denitrifying bacterium[J].Chin J Bioprocess Eng,2019,17(1):104-109..
更新日期/Last Update: 2019-01-30