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[1]余维丽,鹿中华,杨翔,等.肺炎链球菌Spr0982的表达纯化及保守性分析[J].生物加工过程,2017,15(04):64-69.[doi:10.3969/j.issn.1672-3678.2017.04.011]
 YU Weili,LU Zhonghua,YANG Xiang,et al.Expression,purification and conservation analysis of Spr0982 protein from Streptococcus pneumonia R6[J].Chinese Journal of Bioprocess Engineering,2017,15(04):64-69.[doi:10.3969/j.issn.1672-3678.2017.04.011]
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肺炎链球菌Spr0982的表达纯化及保守性分析()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
15
期数:
2017年04期
页码:
64-69
栏目:
出版日期:
2017-07-30

文章信息/Info

Title:
Expression,purification and conservation analysis of Spr0982 protein from Streptococcus pneumonia R6
文章编号:
1672-3678(2017)04-0064-06
作者:
余维丽鹿中华杨翔郑瑶孙昀
安徽医科大学 第二附属医院重症医学科,安徽 合肥 230601
Author(s):
YU WeiliLU ZhonghuaYANG XiangZHENG YaoSUN Yun
Department of Intensive Care Unit,The Second Affiliated Hospital of Anhui Medical University,Hefei 230601,China
关键词:
肺炎链球菌 Spr0982蛋白 重组表达 保守性 耐药性
分类号:
R378.4
DOI:
10.3969/j.issn.1672-3678.2017.04.011
文献标志码:
A
摘要:
肺炎链球菌(Streptococcus pneumoniae)的糖基转移酶是与其耐药性或致病性相关的基因。本文以S. pneumoniae R6 菌株基因组为模板,利用PCR克隆S. pneumoniae中的糖基转移酶(spr0982)基因,构建重组p28(PET28b改造的克隆载体)-Spr0982载体,转化至大肠杆菌BL21-Ril中,利用IPTG诱导表达,并对目的蛋白进行纯化条件的研究。采用ClustalW在线工具对Spr0982蛋白在不同肺炎链球菌菌株中的保守性进行分析。结果显示:p28-Spr0982重组质粒构建成功,经IPTG诱导后于BL21-Ril菌株中获得大量以包涵体形式存在的目的蛋白质; 通过QFF阳离子交换柱和分子筛层析获得较纯的可溶目的蛋白; Spr0982蛋白在不同肺炎链球菌中的保守程度高达99.3%。本研究对于理解S. pneumoniae的致病性和耐药机理研究具有指导意义。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2017-04-14
基金项目:安徽省自然科学基金(1508085QC49); 安徽医科大学校基金(2015XKJ031); 安徽医科大学第二附属医院博士科研基金(2014BKJ034)
作者简介:余维丽(1985—),女,河南商城人,博士,助理研究员,研究方向:微生物生物化学与分子生物学,E-mail:ywl7026@mail.ustc.edu.cn.
更新日期/Last Update: 2017-07-30