|本期目录/Table of Contents|

[1]李凯,杜宗军,林学政.南极细菌Pseudoalteromonas sp. 3-3-1-2产胞外多糖条件优化[J].生物加工过程,2015,13(04):29-35.[doi:10.3969/j.issn.1672-3678.2015.04.006]
 LI Kai,DU Zongjun,LIN Xuezheng.Optimization of exopolysaccharide production by antarctic bacterium Pseudoalteromonas sp. 3-3-1-2[J].Chinese Journal of Bioprocess Engineering,2015,13(04):29-35.[doi:10.3969/j.issn.1672-3678.2015.04.006]
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南极细菌Pseudoalteromonas sp. 3-3-1-2产胞外多糖条件优化()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
13
期数:
2015年04期
页码:
29-35
栏目:
出版日期:
2015-07-30

文章信息/Info

Title:
Optimization of exopolysaccharide production by antarctic bacterium Pseudoalteromonas sp. 3-3-1-2
文章编号:
1672-3678(2015)04-0029-07
作者:
李凯12杜宗军2林学政1
1. 国家海洋局 第一海洋研究所,海洋生物活性物质重点实验室,山东 青岛 266061; 2. 山东大学(威海),海洋学院,山东 威海 264209
Author(s):
LI Kai12 DU Zongjun2 LIN Xuezheng1
1. Key Laboratory of Marine Bioactive Substances,First Institute of Oceanography,State Oceanic Administration, Qingdao 266061,China; 2. Marine College,Shandong University(Weihai),Weihai 264209, China
关键词:
南极细菌 胞外多糖 响应面 Box-Behnken 条件优化
分类号:
TQ929.2
DOI:
10.3969/j.issn.1672-3678.2015.04.006
文献标志码:
A
摘要:
利用单因子试验和响应面法对南极细菌Pseudoalteromonas sp. 3-3-1-2产胞外多糖条件进行了优化。通过单因子试验确定菌株3-3-1-2产胞外多糖的最佳碳源和氮源分别为蔗糖和KNO3,其最佳添加量分别为40 g/L和10 g/L; 最适培养温度为15.0 ℃; 最佳培养基盐度和初始pH分别为4.5%和9; 并确定了对产糖有显著影响的单因素——碳源(蔗糖)、氮源(KNO3)和温度。通过Box-Behnken进行试验设计和Design-Expert响应面软件分析,得到了菌株3-3-1-2产胞外多糖发酵条件的优化条件:蔗糖43.1 g/L、KNO3 9.6 g/L和温度17.2 ℃。在此优化条件下,菌株3-3-1-2发酵液的粗胞外多糖产量可达3.03 g/L。

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备注/Memo

备注/Memo:
收稿日期:2014-07-29
基金项目:海洋公益性行业研究专项(201205020)
作者简介:李凯(1988—),男,山东聊城人,硕士研究生,研究方向:生物多糖; 林学政(联系人),研究员,E-mail:linxz@fio.org.cn.
更新日期/Last Update: 2015-07-30