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[1]商静生,纪晓俊,聂志奎,等.产花生四烯酸油脂高山被孢霉干法造粒提油及菌粕的重复利用[J].生物加工过程,2015,13(02):24-29.[doi:10.3969/j.issn.1672-3678.2015.02.005]
 SHANG Jingsheng,JI Xiaojun,NIE Zhikui,et al.Extraction of lipids from arachidonic acid-rich oil producing Mortierella alpina with dry granulation and reusing fungal residues[J].Chinese Journal of Bioprocess Engineering,2015,13(02):24-29.[doi:10.3969/j.issn.1672-3678.2015.02.005]
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产花生四烯酸油脂高山被孢霉干法造粒提油及菌粕的重复利用()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
13
期数:
2015年02期
页码:
24-29
栏目:
出版日期:
2015-03-30

文章信息/Info

Title:
Extraction of lipids from arachidonic acid-rich oil producing Mortierella alpina with dry granulation and reusing fungal residues
文章编号:
1672-3678(2015)02-0024-06
作者:
商静生纪晓俊聂志奎张瑷珲颜佳铖张鑫黄和
南京工业大学 生物与制药工程学院 材料化学工程国家重点实验室,江苏 南京 210009
Author(s):
SHANG JingshengJI XiaojunNIE ZhikuiZHANG AihuiYAN JiachengZHANG XinHANG He
State Key Laboratory of Materials-Oriented Chemical Engineering,College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University,Nanjing 210009,China
关键词:
花生四烯酸 高山被孢霉 干法造粒 菌粕 生产强度
分类号:
TS224.4;Q815
DOI:
10.3969/j.issn.1672-3678.2015.02.005
文献标志码:
A
摘要:
对高产花生四烯酸油脂高山被孢霉干法造粒提油工艺中3个参数及菌粕重复利用进行研究。设计三因素四水平L16(43)正交试验,考察造粒直径、正己烷与菌粕的比例、萃取时间对提油效率及正己烷回收率的影响。结果表明:当含水率20%~25%、造粒直径1.2 mm、正己烷的体积与颗粒的质量比20:1 L/g、萃取时间5 h时,每克干菌体油脂得率0.559 4 g,正己烷的回收率达90%。与均质湿法提油相比,分别提高了17.35%和83.67%。用碱性蛋白酶及复合酶(纤维素酶、果胶酶和碱性蛋白酶)处理菌粕,以50%替代氮源(酵母膏)添加到培养基中,发酵周期分别为10 d和7 d。此时,生物量、油脂产量、花生四烯酸产量都达到了最大值,分别为30.33 g/L、16.25 g/L、8.59 g/L(碱性蛋白酶处理); 29.77 g/L、16.89 g/L、7.12 g/L(复合酶处理)。其中,复合酶处理菌粕的生产强度可达4.253 g/(L·d)、2.413 g/(L·d)和1.017 g/(L·d),与碱性蛋白酶处理相比,其生产强度分别提高了40.22%、48.49%和18.39%。干法造粒提油是有效的,且酶法处理菌粕的方法有应用前景。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2014-03-07
基金项目:国家高技术研究发展计划(863计划)(2014AA021703); 国家自然科学基金(21376002、 21476111); 江苏省自然科学基金(BK20131405); 江苏省高校优势学科建设工程
作者简介:商静生(1987—),男,河北保定人,硕士研究生,研究方向:生物化工; 纪晓俊(联系人),副教授,E-mail:xiaojunji@njtech.edu.cn.
更新日期/Last Update: 2015-03-30