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[1]代军,赵根海,王鹏,等.高山被孢霉发酵生产花生四烯酸的宏观形态[J].生物加工过程,2014,12(03):47-51.[doi:10.3969/j.issn.1672-3678.2014.03.009]
 DAI Jun,ZHAO Genhai,WANG Peng,et al.Macro-morphology characterization of Mortierella alpina during the cultivation[J].Chinese Journal of Bioprocess Engineering,2014,12(03):47-51.[doi:10.3969/j.issn.1672-3678.2014.03.009]
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高山被孢霉发酵生产花生四烯酸的宏观形态()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
12
期数:
2014年03期
页码:
47-51
栏目:
出版日期:
2014-05-30

文章信息/Info

Title:
Macro-morphology characterization of Mortierella alpina during the cultivation
文章编号:
1672-3678(2014)03-0047-05
作者:
代军1赵根海1王鹏1刘会1宋均营1胡以华2郑之明1
1.中国科学院 合肥物质科学研究院 离子束生物工程学重点实验室,合肥 230031; 2.解放军电子工程学院,合肥 230037
Author(s):
DAI Jun1ZHAO Genhai1WANG Peng1LIU Hui1SONG Junying1HU Yihua2ZHENG Zhiming1
1.Key Laboratory of Ion Beam Bioengineering,Hefei Institutes of Physical Science,Chinese Academy of Science, Hefei 230031,China; 2.Electronics Engineering Institute,Hefei 230037,China
关键词:
花生四烯酸(ARA) 图像处理 客观形态 高山被孢霉
分类号:
Q934.1
DOI:
10.3969/j.issn.1672-3678.2014.03.009
文献标志码:
A
摘要:
采用图像处理技术对高山被孢霉(Mortierella alpina)发酵过程中的不同形态进行分析,并对其产花生四烯酸(ARA)能力进行了比较。研究发现:复合N源中蛋白胨与酵母粉比例是影响高山被孢霉宏观形态的重要因素,球形形态生长的菌体中ARA产量较分散,菌丝体中ARA产量高; 在球形形态中,空心球的菌体生物量低,ARA比例低,蓬松球可以兼顾菌体高生物量、高油脂比例及高ARA比例。产ARA能力由生物量、油脂比例及油脂中ARA比例共同决定。结果表明:直径大约4 mm、成核区域面积大约为43.6%、紧密度为71.36的蓬松球形态,是高山被孢霉一种相对较佳的发酵形态,其菌体产ARA能力分别是空心球和分散丝状菌体产ARA能力的2.01和2.70倍。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2013-04-07
基金项目:国家高技术研究发展计划(863计划)(2011ARA02A202); 安徽省自然科学基金(1308085MA07)
作者简介:代军(1989—)男,四川广安人,硕士研究生,研究方向:工业微生物; 郑之明(联系人),研究员,博士生导师,E-mail:zmzheng@ipp.ac.cn; 赵根海(联系人),博士,助理研究员,E-mail:zhgh327@126.com.
更新日期/Last Update: 2014-05-30