|本期目录/Table of Contents|

[1]钟耀华,钱远超,任美斌,等.丝状真菌降解转化纤维素的机制与遗传改良前景[J].生物加工过程,2014,12(01):46-54.[doi:10.3969/j.issn.1672-3678.2014.01.008]
 ZHONG Yaohua,QIAN Yuanchao,REN Meibin,et al.Progress in bioconversion of cellulose by filamentous fungi and related genetic engineering strategies[J].Chinese Journal of Bioprocess Engineering,2014,12(01):46-54.[doi:10.3969/j.issn.1672-3678.2014.01.008]
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丝状真菌降解转化纤维素的机制与遗传改良前景()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
12
期数:
2014年01期
页码:
46-54
栏目:
出版日期:
2014-01-20

文章信息/Info

Title:
Progress in bioconversion of cellulose by filamentous fungi and related genetic engineering strategies
文章编号:
1672-3678(2014)01-0046-09
作者:
钟耀华钱远超任美斌汪天虹
山东大学 生命科学学院 微生物技术国家重点实验室,济南 250100
Author(s):
ZHONG YaohuaQIAN YuanchaoREN MeibinWANG Tianhong
State Key Laboratory of Microbial Technology,School of Life Sciences,Shandong University,Jinan 250100,China
关键词:
纤维素酶 丝状真菌 遗传改良
分类号:
Q78
DOI:
10.3969/j.issn.1672-3678.2014.01.008
文献标志码:
A
摘要:
丝状真菌可以分泌大量纤维素酶及辅助酶来降解纤维素底物,也是目前工业上纤维素酶的主要生产者。回顾并综述了丝状真菌降解转化纤维素的酶系和机制进展,详细总结了组学研究在纤维素酶研究上的新成果,并探讨了提高丝状真菌酶系效率和产量的遗传改良策略。

参考文献/References:

[1] Kubicek C P,Mikus M,Schuster A,et al.Metabolic engineering strategies for the improvement of cellulase production by Hypocrea jecorina[J].Biotechnol Biofuels,2009,2:19.doi:10.1186/1754-6834-2-19.
[2] Chandel A K,Chandrasekhar G,Silva M B,et al.The realm of cellulases in biorefinery development[J].Crit Rev Biotechnol,2012,32(3):187-202.
[3] Regalbuto J R.Cellulosic biofuels:got gasoline[J].Science,2009,325:822-824.
[4] Lynd L R,van Zyl W H,McBride J E,et al.Consolidated bioprocessing of cellulosic biomass:an update[J].Curr Opin Biotechnol,2005,16(5):577-583.
[5] Greer D.Spinning straw into fuel[J].Biocycle,2005,46(4):61-65.
[6] Wilson D B.Cellulases and biofuels[J].Curr Opin Biotechnol,2009,20(3):295-299.
[7] Percival Zhang Y H,Himmel M E,Mielenz J R.Outlook for cellulase improvement:screening and selection strategies[J].Biotechnol Adv,2006,24(5):452-481.
[8] Himmel M E,Ding S Y,Johnson D K,et al.Biomass recalcitrance:engineering plants and enzymes for biofuels production[J].Science,2007,315:804-807.
[9] Martinez D,Berka R M,Henrissat B,et al.Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei(syn.Hypocrea jecorina)[J].Nat Biotechnol,2008,26(5):553-560.
[10] Liu G,Zhang L,Wei X,et al.Genomic and secretomic analyses reveal unique features of the lignocellulolytic enzyme system ofPenicillium decumbens[J].PLoS One,2013,8(2):e55185.doi:10.1371/journal.pone.0055185.
[11] Lynd L R,Weimer P J,van Zyl W H,et al.Microbial cellulose utilization:fundamentals and biotechnology[J].Microbiol Mol Biol Rev,2002,66(3):506-577.
[12] Margeot A,Hahn-H?gerdal B,Edlund M,et al.New improvements for lignocellulosic ethanol[J].Curr Opin Biotechnol,2009,20(3):372-380.
[13] Gusakov A V.Alternatives to Trichoderma reesei in biofuel production[J].Trends Biotechnol,2011,29(9):419-425.
[14] Mandels M,Sternberg D.Recent advances in cellulase technology[J].J Ferment Technol,1976,54(4):267-286.
[15] de Vries R P,Visser J.Aspergillus enzymes involved in degradation of plant cell wall polysaccharides[J].Microb Mol Biol Rev,2001,65(4):497-522.
[16] Schülein M.Enzymatic properties of cellulases from Humicola insolens[J].J Biotechnol,1997,57(1/2/3):71-81.
[17] Berlin A,Gilkesa N,Kilburn D,et al.Evaluation of novel fungal cellulase preparations for ability to hydrolyze softwood substrates:evidence for the role of accessory enzymes[J].Enzyme Microb Technol,2005,37(2):175-184.
[18] Morozova V V,Gusakov A V,Andrianov R M,et al.Cellulases of Penicillium verruculosum[J].Biotechnol J,2010,5(8):871-880.
[19] Bhiri F,Gargourib A,Ali M,et al.Molecular cloning,gene expression analysis and structural modeling of the cellobiohydrolase I from Penicillium occitanis[J].Enzyme Microb Technol,2010,46(2):74-81.
[20] Ikeda,Y,Hayashi H,Okuda N,et al.Efcient cellulase production by the lamentous fungus Acremonium cellulolyticus[J].Biotechnol Prog,2007,23(2):333-338.
[21] Fujii T,Fang X,Inoue H,et al.Enzymatic hydrolyzing performance of Acremonium cellulolyticus and Trichoderma reesei against three lignocellulosic materials[J].Biotechnol Biofuels,2009,2(1):24.doi:10.1186/1754-6834-2-24.
[22] Voutilainen S P,Puranen T,Siika-Aho M,et al.Cloning,expression,and characterization of novel thermostable family 7 cellobiohydrolases[J].Biotechnol Bioeng,2008,101(3):515-528.
[23] Gusakov A V,Salanovich T N,Antonov A I,et al.Design of highly efcient cellulasemixtures for enzymatic hydrolysis of cellulose[J].Biotechno Bioeng,2007,97(5):1028-1038.
[24] Bayer E A,Chanzy H,Lamed R,et al.Cellulose,cellulases and cellulosomes[J].Curr Opin Struct Biol,1998,8(5):548-557.
[25] Baldrian P,Valásková V.Degradation of cellulose by basidiomycetous fungus[J].FEMS Microbiol Rev,2008,32(3):501-521.
[26] Sukharnikov L O,Cantwell B J,Podar M,et al.Cellulases:ambiguous nonhomologous enzymes in a genomic perspective[J].Trends Biotechnol,2011,29(10):473-479.
[27] Morais H,Ramos C,Matos N,et al.Liquid chromatographic and electrophoretic characterisation of extracellular beta-glucosidase of Pleurotus ostreatus grown in organic waste[J].J Chromatogr B,2002,770:111-119.
[28] Ishikawa E,Sakai T,Ikemura H,et al.Identification,cloning,and characterization of a Sporobolomyces singularis beta-galactosidase-like enzyme involved in galacto-oligosaccharide production[J].J Biosci Bioeng,2005,99:331-339.
[29] Dashtban M,Schraft H,Qin W.Fungal bioconversion of lignocellulosic residues:opportunities & perspectives[J].Int J Biol Sci,2009,5(6):578-595.
[30] Kubicek C P.Release of carboxymethyl-cellulase and β-glucosidase from cell walls of Trichoderma reesei[J].Eur J Appl Microbiol Biotechnol,1981,13(4):226-231.
[31] Reese E T.Enzymatic hydrolysis of cellulose[J].Appl Microbiol,1956,4(1):39-45.
[32] Merino S T,Cherry J.Progress and challenges in enzyme development for biomass utilization[J].Adv Biochem Eng Biotechnol,2007,108:95-120.
[33] Horn S J,Vaaje-Kolstad G,Westereng B,et al.Novel enzymes for the degradation of cellulose[J].Biotechnol Biofuels,2012,5(1):45.doi:10.1186/1754-6834-5-45.
[34] Vaaje-Kolstad G,Westereng B,Horn S J,et al.An oxidative enzyme boosting the enzymatic conversion of recalcitrant polysaccharides[J].Science,2010,330:219-222.
[35] Foreman P K,Brown D,Dankmeyer L,et al.Transcriptional regulation of biomass-degrading enzymes in the lamentous fungus Trichoderma reesei[J].J Biol Chem,2003,278:31988-31997.
[36] Peterson R,Nevalainen H.Trichoderma reesei RUT-C30:thirty years of strain improvement[J].Microbiology,2012,158(1):58-68.
[37] Liu G,Qin Y,Li Z,et al.Development of highly efficient,low-cost lignocellulolytic enzyme systems in the post-genomic era[J].Biotechnol Adv,2013,31(6):962-975.
[38] Chundawat S P,Lipton M S,Purvine S O,et al.Proteomics-based compositional analysis of complex cellulase-hemicellulase mixtures[J].J Proteome Res,2011,10(10):4365-4372.
[39] Phillips C M,Iavarone A T,Marletta M A.Quantitative proteomic approach for cellulosedegradation by Neurospora crassa[J].J Proteome Res,2011,10(9):4177-4185.
[40] Ravalason H,Grisel S,Chevret D,et al.Fusarium verticillioides secretome as a source of auxiliary enzymes to enhance saccharication of wheat straw[J].Bioresour Technol,2012,114:589-596.
[41] Couturier M,Navarro D,Olive C,et al.Post-genomic analyses of fungal lignocellulosic biomass degradation reveal the unexpected potential of the plant pathogen Ustilago maydis[J].BMC Genomics,2012,13:57.doi:10.1186/1471-2164-13-57.
[42] Berrin J G,Navarro D,Couturier M,et al.Exploring the natural fungal biodiversity of tropical and temperate forests toward improvement of biomass conversion[J].Appl Environ Microbiol,2012,78:6483-6490.
[43] Ma L,Zhang J,Zou G,et al.Improvement of cellulase activity in Trichoderma reesei by heterologous expression of a beta-glucosidase gene from Penicillium decumbens[J].Enzyme Microb Technol,2011,49(4):366-371.
[44] Rahman Z,Shida Y,Furukawa T,et al.Application of Trichoderma reesei cellulase and xylanase promoters through homologous recombination for enhanced production of extracellular beta-glucosidase I[J].Biosci Biotechnol Biochem,2009,73(5):1083-1089.
[45] Bey M,Berrin J G,Poidevin L,et al.Heterologous expression of Pycnoporus cinnabarinus cellobiose dehydrogenase in Pichia pastoris and involvement in saccharication processes[J].Microb Cell Fact,2011,10:113.doi:10.1186/1475-2859-10-113.
[46] Couturier M,Haon M,Coutinho P M.Podospora anserina hemicellulases potentiate the Trichoderma reesei secretome for saccharication of lignocellulosic biomass[J].Appl Environ Microbiol,2011,77(1):237-246.
[47] Banerjee G,Car S,Scott-Craig J S,et al.Synthetic multi-component enzyme mixtures for deconstruction of lignocellulosic biomass[J].Bioresour Technol,2010,101(23):9097-9105.
[48] 钟耀华,王晓利,汪天虹.丝状真菌高效表达异源蛋白研究进展[J].生物工程学报,2008,24(4):531-540.
[49] Zhang J,Zhong Y,Zhao X,et al.Development of the cellulolytic fungus Trichoderma reesei strain with enhanced beta-glucosidase and lter paper activity using strong articial cellobiohydrolase 1 promoter[J].Bioresour Technol,2010,101(24):9815-9818.
   [50] Murray P,Aro N,Collins C,et al.Expression in Trichoderma reesei and characterisation of a thermostable family 3 beta-glucosidase from the moderately thermophilic fungus Talaromyces emersonii[J].Protein Expr Purif,2004,38(2):248-257.
[51] Miettinen-Oinonen A,Paloheimo M,Lantto R,et al.Enhanced production of cellobiohydrolases in Trichoderma reesei and evaluation of the new preparations in biofinishing of cotton[J].J Biotechnol,2005,116(3):305-317.
[52] Bower B,Larenas E,Mitchinson C.Exo-endo cellulase fusion protein:WO,20060057672[P].2006-03-16.
[53] Alper H,Moxley J,Nevoigt E,et al.Engineering yeast transcription machinery for improved ethanol tolerance and production[J].Science,2006,314:1565-1568.
[54] Valkonen M,Ward M,Wang H,et al.Improvement of foreign-protein production in Aspergillus niger var.awamori by constitutive induction of the unfolded-protein response[J].Appl Environ Microbiol,2003,69(12):6979-6986.
[55] Idiris A,Tohda H,Kumagai H,et al.Engineering of protein secretion in yeast:strategies and impact on protein production[J].Appl Microbiol Biotechnol,2010,86:403-417.
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备注/Memo

备注/Memo:
收稿日期:2013-11-03
基金项目:国家重点基础研究发展计划(973 计划)(2011CB707403); 国家自然科学基金(31070036,31370135); 山东省优秀中青年科学家科研奖励基金(BS2011SW017); 山东大学自主创新基金(2012TS012)
作者简介:钟耀华(1979—),男,山东临朐人,副教授,博士,研究方向:真菌蛋白分泌机制与纤维素酶系改良工程,E-mail:zhongyaohua@sdu.edu.cn.
更新日期/Last Update: 2014-01-30