|本期目录/Table of Contents|

[1]刘欣,傅宁华,张红漫,等.高山被孢霉淬灭方法及胞内代谢产物的气相色谱-质谱分析比较[J].生物加工过程,2013,11(06):63-67.[doi:10.3969/j.issn.1672-3678.2013.06.013]
 LIU Xin,FU Ninghua,ZHANG Hongman,et al.Comparison of sampling method of intracellular metabolites in Mortierella alpina and the analysis of the metabolites by GC-MS[J].Chinese Journal of Bioprocess Engineering,2013,11(06):63-67.[doi:10.3969/j.issn.1672-3678.2013.06.013]
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高山被孢霉淬灭方法及胞内代谢产物的气相色谱-质谱分析比较()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
11
期数:
2013年06期
页码:
63-67
栏目:
出版日期:
2013-11-30

文章信息/Info

Title:
Comparison of sampling method of intracellular metabolites in Mortierella alpina and the analysis of the metabolites by GC-MS
文章编号:
1672-3678(2013)06-0063-05
作者:
刘欣1傅宁华1张红漫2纪晓俊1张鑫2杨健健1黄和1
1.南京工业大学 生物与制药工程学院 材料化学工程国家重点实验室,南京 210009; 2.南京工业大学 理学院,南京 210009
Author(s):
LIU Xin1FU Ninghua1ZHANG Hongman2JI Xiaojun1ZHANG Xin2YANG Jianjian1HUANG He1
1.State Key Laboratory of Materials-Oriented Chemical Engineering,College of Biotechnology and Pharmaceutical Engineering,Nanjing University of Technology,Nanjing 210009; 2.College of Sciences,Nanjing University of Technology,Nanjing 10009
关键词:
花生四烯酸 高山被孢霉 代谢组学 代谢产物 取样 GC-MS分析
分类号:
S182
DOI:
10.3969/j.issn.1672-3678.2013.06.013
文献标志码:
A
摘要:
在花生四烯酸生产菌高山被孢霉代谢组学研究中,需利用胞内代谢物的提取手段并基于气相色谱-质谱(GC-MS)分析方法对其进行检测。比较了3种胞内代谢物提取方法及不同色谱柱条件下GC-MS分析结果。研究表明:采用冷甲醇淬灭分别较液氮直接淬灭及真空过滤后,减少了胞内代谢物的泄露并更好地实现了胞外及胞内代谢物的分离。在对代谢物分析的比较中,极性色谱柱(DB-FFAP)检出的代谢物仅为11种,主要为有机酸、醛类; 而代谢物经衍生化后采用非极性色谱柱(DB-5)共检出32种化合物,主要为糖、糖苷及醇类。

参考文献/References:

[1] 袁成凌,姚建铭,余增亮.花生四烯酸及其代谢物的生物学作用[J].中国药物化学杂志,2000,10(1):75-78.
[2] Gill I,Valivety R.Polyunsaturated fatty acids:1.occurrence,biological activities and applications[J].Trends Biotechnol,1997,15(10):401-409.
[3] Fridman E,Pichersky E.Metabolomics,genomics,proteomics,and the identification of enzymes and their substrates and products[J].Curr Opin Plant Biol,2005,8(3):242-248.
[4] Gummer J P A,Krill C,Du Fall L,et al.Metabolomics protocols for filamentous fungi[C]//Bolton M D D,Thomma,B P H J P.Plant fungal pathogens:methods and protocols.New York:Humana Press,2012:237-254.
[5] Hajjaj H,Blanc P J,Goma G,et al.Sampling techniques and comparative extraction procedures for quantitative determination of intra-and extracellular metabolites in filamentous fungi[J].FEMS Microbiol Lett,1998,164(1):195-200.
[6] Smart K F,Aggio R B M,van Houtte J R,et al.Analytical platform for metabolome analysis of microbial cells using methyl chloroformate derivatization followed by gas chromatography-mass spectrometry[J].Nat Protoc,2010,5(10):1709-1729.
[7] Wittmann C,Kr?mer J O,Kiefer P,et al.Impact of the cold shock phenomenon on quantification of intracellular metabolites in bacteria[J].Anal Biochem,2004,327(1):135-139.
[8] Gonzalez B,Francois J,Renaud M.A rapid and reliable method for metabolite extraction in yeast using boiling buffered ethanol[J].Yeast,1997,13(14):1347-1355.
[9] Ruijter G J G,Visser J.Determination of intermediary metabolites in Aspergillus niger[J].J Microbiol Methods 1996,25(3):295-302.
[10] Ding M Z,Wang X,Yang Y,et al.Comparative metabolic profiling of parental and inhibitors-tolerant yeasts during lignocellulosic ethanol fermentation[J].Metabolomics,2011,8(2):232-243.
[11] Link H,Anselment B,Weuster-Botz D.Leakage of adenylates during cold methanol/glycerol quenching of Escherichia coli[J].Metabolomics,2008,4(3):240-247.

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备注/Memo

备注/Memo:
收稿日期:2012-09-29
基金项目:国家自然科学基金(21176124); 国家自然科学基金重点项目(20936002); 国家科技支撑计划(2011BAD23B03); 江苏省高校优势学科建设工程
作者简介:刘欣(1983—),女,吉林长春人,博士研究生,研究方向:微生物发酵与代谢工程; 黄和(联系人):教授,E-mail:biotech@njut.edu.cn.
更新日期/Last Update: 2013-11-30