|本期目录/Table of Contents|

[1]陈睿,孙润广,王小梅,等.猪肚菇多糖WPG1的相对分子质量测定及原子力显微镜观测[J].生物加工过程,2013,11(05):61-66.[doi:10.3969/j.issn.1672-3678.2013.05.012]
 CHEN Rui,SUN Runguang,WANG Xiaomei,et al.Composition of monosaccharide and AFM analysis of WPG1 from Panus giganteus polysaccharides[J].Chinese Journal of Bioprocess Engineering,2013,11(05):61-66.[doi:10.3969/j.issn.1672-3678.2013.05.012]
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猪肚菇多糖WPG1的相对分子质量测定及原子力显微镜观测()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
11
期数:
2013年05期
页码:
61-66
栏目:
出版日期:
2013-09-30

文章信息/Info

Title:
Composition of monosaccharide and AFM analysis of WPG1 from Panus giganteus polysaccharides
文章编号:
1672-3678(2013)05-0061-06
作者:
陈睿1孙润广1王小梅1张化朋2
1. 陕西师范大学 物理学与信息技术学院, 西安 710062; 2. 陕西师范大学 食品工程与营养科学学院,西安 710062
Author(s):
CHEN Rui1 SUN Runguang1 WANG Xiaomei1 ZHANG Huapeng2
1. College of Physics and Information Technology, Shaanxi Normal University, Xi’an 710062,China 2. College of Food Engineering and Nutrition Science, Shaanxi Normal University, Xi’an 710062,China
关键词:
猪肚菇 多糖 原子力显微镜 色谱
分类号:
S646
DOI:
10.3969/j.issn.1672-3678.2013.05.012
文献标志码:
A
摘要:
热水提取猪肚菇粗多糖中的酸性多糖WPG1,经凝胶纯化后用高效液相色谱(HPLC)测定其相对分子质量,用气相色谱对其单糖组成进行分析,用原子力显微镜(AFM)对其形态结构进行观测。结果表明:酸性多糖WPG1经SephacrylS-300凝胶层析纯化后得到WPG1-1和WPG1-2这2个组分; 经HPLC分析可知WPG1-1与WPG1-2均为单一组分,其相对分子质量分别为1.7×106和1.6×104; WPG1-1与WPG1-2的单糖组成主要是糖醛酸、甘露糖、葡萄糖和半乳糖; 原子力显微镜观测图分析表明WPG1-1为网链状聚集体结构,WPG1-2呈梭状聚集体,两者均非单链存在。

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备注/Memo

备注/Memo:
收稿日期:2012-10-16
基金项目:国家自然科学基金(10874108); 陕西省自然科学基础研究计划(SJ08A16)
作者简介:陈睿(1988—),女,陕西榆林人,硕士研究生,研究方向:天然产物有效成分的化学研究; 孙润广(联系人),教授,E-mail:sunrunguang@snnu.edu.cn.
更新日期/Last Update: 2013-09-30