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[1]王珊珊,高璐,严明,等.过表达氨基葡萄糖脱氨酶对大肠杆菌氨基葡萄糖合成及中心碳代谢的影响[J].生物加工过程,2013,11(04):42-48.[doi:10.3969/j.issn.1672-3678.2013.04.008]
 WANG Shanshan,GAO Lu,YAN Ming,et al.Effects of overexpression glucosamine deaminase on glucosamine synthesis and central carbon metabolism in Escherichia coli[J].Chinese Journal of Bioprocess Engineering,2013,11(04):42-48.[doi:10.3969/j.issn.1672-3678.2013.04.008]
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过表达氨基葡萄糖脱氨酶对大肠杆菌氨基葡萄糖合成及中心碳代谢的影响()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
11
期数:
2013年04期
页码:
42-48
栏目:
出版日期:
2013-07-30

文章信息/Info

Title:
Effects of overexpression glucosamine deaminase on glucosamine synthesis and central carbon metabolism in Escherichia coli
文章编号:
1672-3678(2013)04-0042-07
作者:
王珊珊高璐严明许琳
南京工业大学 生物与制药工程学院,南京 211800
Author(s):
WANG ShanshanGAO LuYAN MingXU Lin
College of Biotechnology and Pharmaceutical Engineering,Nanjing University of Technology,Nanjing 211800,China
关键词:
大肠杆菌 脱氨酶 氨基葡萄糖
分类号:
Q78
DOI:
10.3969/j.issn.1672-3678.2013.04.008
文献标志码:
A
摘要:
考察过表达氨基葡萄糖脱氨酶对氨基葡萄糖合成及大肠杆菌(Escherichia coli)中心碳代谢的影响。实验结果表明:过表达氨基葡萄糖脱氨酶使得在36 g/L葡萄糖,pH为9.0的发酵条件下,发酵24 h后,重组菌发酵液中氨基葡萄糖、丙酮酸和乙酸的量分别是对照菌Rosetta的2.1、1.48和1.74倍; 而乳酸的量为2.53 g/L,对照菌Rosetta发酵液中的乳酸含量未检测到,重组菌发酵液中柠檬酸及α-酮戊二酸的含量分别是Rosetta的2.99和2.73倍。

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备注/Memo

备注/Memo:
收稿日期:2012-03-27
基金项目:国家高技术研究发展计划(863计划)(2012AA022101)
作者简介:王珊珊(1988—),女,河南洛阳人,硕士研究生,研究方向:生物化工; 严明(联系人),副教授,E-mail:yanming@njut.edu.cn.
更新日期/Last Update: 2013-07-30