|本期目录/Table of Contents|

[1]彭超,黄和,纪晓俊,等.利用甘蔗糖蜜发酵生产花生四烯酸的研究[J].生物加工过程,2013,11(03):1-6.[doi:10.3969/j.issn.1672-3678.2013.03.001]
 PENG Chao,HUANG He,JI Xiaojun,et al.Arachidonic acid production from cane molasses by Mortierella alpina[J].Chinese Journal of Bioprocess Engineering,2013,11(03):1-6.[doi:10.3969/j.issn.1672-3678.2013.03.001]
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利用甘蔗糖蜜发酵生产花生四烯酸的研究()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
11
期数:
2013年03期
页码:
1-6
栏目:
出版日期:
2013-05-30

文章信息/Info

Title:
Arachidonic acid production from cane molasses by Mortierella alpina
文章编号:
1672-3678(2013)03-0001-06
作者:
彭超1黄和12纪晓俊1刘欣1聂志奎1邓中涛1
1. 南京工业大学 生物与制药工程学院,南京 211800; 2. 南京工业大学 材料化学工程国家重点实验室,南京 210009
Author(s):
PENG Chao1HUANG He12JI Xiaojun1LIU Xin1NIE Zhikui1DENG Zhongtao1
1. College of Biotechnology and Pharmaceutical Engineering,Nanjing University of Technology,Nanjing 211800,China; 2. State Key Laboratory of Materials-Oriented Chemical Engineering,Nanjing University of Technology,Nanjing 210009,China
关键词:
花生四烯酸 糖蜜:发酵 高山被孢霉
分类号:
Q935
DOI:
10.3969/j.issn.1672-3678.2013.03.001
文献标志码:
A
摘要:
通过培养高山被孢霉利用糖蜜来发酵生产花生四烯酸(ARA),研究了不同甘蔗糖蜜预处理方法对ARA发酵生产的影响。研究表明:H2SO4法是最利于ARA发酵生产的糖蜜预处理方法。利用预处理的甘蔗糖蜜发酵生产ARA,通过单因素实验设计,确定了最优的培养条件,包括初始还原糖 80 g/L,N源6 g/L,接种量20%,初始pH6.0和培养温度25 ℃,在此条件下发酵,干细胞质量、油脂含量、ARA产量和糖利用率分别达到28.5 g/L、11.7 g/L、3.68 g/L和94.5%。

参考文献/References:

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[11] 董晋军,郑璞,孙志浩.利用甘蔗糖蜜半连续发酵生产琥珀酸[J].化工学报,2008,59(6):1490-1495.
[12] 杨卓娜,李建,黄秀梅,等.利用甘蔗糖蜜厌氧发酵产丁二酸的研究[J].中国酿造,2010(5):35-38.
[13] Johnson V,Singh M,Saini V S,et al.Effect of pH on lipid accumulation by an oleaginous yeast:Rhodotorula glutinis IIP-30[J].World J Microbiol Biotechnol,1992,8(4):382-384.
[14] Peng C,Huang H,Ji X,et al.A temperature-shift strategy for efficient arachidonic acid fermentation by Mortierella alpina in batch culture[J].Biochem Eng J,2010,53(1):92-96.

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备注/Memo

备注/Memo:
收稿日期:2012-03-27
基金项目:江苏省六大人才高峰项目(2008-D-63); 江苏省研究生创新计划(CX10B_175Z)
作者简介:彭超(1985—),男,湖南汉寿人,博士,研究方向:微生物发酵与代谢工程; 黄和(联系人),教授,E-mail:biotech@njut.edu.cn.
更新日期/Last Update: 2013-03-30