|本期目录/Table of Contents|

[1]梁大勇,黄芳,赵晨,等.桑黄粗多糖除蛋白及抗肿瘤活性[J].生物加工过程,2012,10(03):56-60.[doi:10.3969/j.issn.1672-3678.2012.03.011]
 LIANG Dayong,HUANG Fang,ZHAO Chen,et al.Deproteinization and antitumor activity of Phellinus igniarius crude polysaccharides[J].Chinese Journal of Bioprocess Engineering,2012,10(03):56-60.[doi:10.3969/j.issn.1672-3678.2012.03.011]
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桑黄粗多糖除蛋白及抗肿瘤活性()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
10
期数:
2012年03期
页码:
56-60
栏目:
出版日期:
2012-05-30

文章信息/Info

Title:
Deproteinization and antitumor activity of Phellinus igniarius crude polysaccharides
文章编号:
1672-3678(2012)03-0056-05
作者:
梁大勇黄芳赵晨于朋秦丹宋爱荣
青岛农业大学 山东省应用真菌重点实验室,青岛 266109
Author(s):
LIANG DayongHUANG FangZHAO ChenYU PengQIN DanSONG Airong
Shandong Provincial Laboratory of Applied Mycology, Qingdao Agricultural University, Qingdao 266109, China
关键词:
桑黄 多糖 Sevag法
分类号:
Q939.9
DOI:
10.3969/j.issn.1672-3678.2012.03.011
文献标志码:
A
摘要:
旨在研究Sevag法除桑黄粗多糖中蛋白的最佳工艺,并通过噻唑蓝(MTT)细胞比色法测定除蛋白前后桑黄多糖的抗肿瘤活性。在单因素试验Sevag用量、氯仿与正丁醇体积比、萃取次数、震荡时间的基础上,设计正交试验,经过方差分析,确定Sevag法除桑黄粗多糖蛋白的最佳方案; 并且对除蛋白后多糖与粗多糖进行体外抗肿瘤实验。Sevag法除桑黄粗多糖蛋白的最佳工艺:Sevag用量50 mL、V(氯仿)/V(正丁醇)=3、最佳萃取次数6次、最佳振荡时间20 min,在此条件下,多糖的得率为64.8%。体外细胞试验表明除蛋白后多糖与粗多糖对肿瘤细胞EC109和SiHa的抑制率没有明显差异。

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备注/Memo

备注/Memo:
收稿日期:2011-09-23
基金项目:山东省科技攻关资助项目(2010GNC10956)
作者简介:梁大勇(1983—),男,山东威海人,硕士研究生,研究方向:微生物学; 宋爱荣(联系人),研究员,E-mail:airongsong@163.com.
更新日期/Last Update: 2012-05-30