|本期目录/Table of Contents|

[1]刘嵘明,梁丽亚,王光明,等.基于辅因子调控对大肠杆菌两阶段发酵产丁二酸的影响[J].生物加工过程,2012,10(03):1-5.[doi:10.3969/j.issn.1672-3678.2012.03.001]
 LIU Rongming,LIANG Liya,WANG Guangming,et al.Effect of cofactor regulation on succinate production in Escherichia coli during dual-phase fermentation[J].Chinese Journal of Bioprocess Engineering,2012,10(03):1-5.[doi:10.3969/j.issn.1672-3678.2012.03.001]
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基于辅因子调控对大肠杆菌两阶段发酵产丁二酸的影响()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
10
期数:
2012年03期
页码:
1-5
栏目:
出版日期:
2012-05-30

文章信息/Info

Title:
Effect of cofactor regulation on succinate production in Escherichia coli during dual-phase fermentation
文章编号:
1672-3678(2012)03-0001-05
作者:
刘嵘明梁丽亚王光明张敏马江锋陈可泉姜岷韦萍
南京工业大学 生物与制药工程学院,南京 210009
Author(s):
LIU RongmingLIANG LiyaWANG GuangmingZHANG MinMA JiangfengCHEN KequanJIANG MinWEI Ping
College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China
关键词:
大肠杆菌 烟酸 转磷酸核糖激酶 丁二酸
分类号:
Q815
DOI:
10.3969/j.issn.1672-3678.2012.03.001
文献标志码:
A
摘要:
考察了E.coli NZN111及其重组菌株E.coli NZN111/pTrc99a-pncB发酵生产丁二酸的性能。E.coli NZN111两阶段发酵丁二酸的同时,会造成丙酮酸的大量积累。研究发现:通过过量表达烟酸转磷酸核糖激酶,两阶段发酵重组菌株E.coli NZN111/pTrc99a-pncB,减少丙酮酸的积累且无副产物乙酸生成,提高丁二酸的产量,丁二酸得率和耗糖速率分别提高了139%和20%。

参考文献/References:

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[6] 刘嵘明,马江锋,梁丽亚,等.过量表达烟酸转磷酸核糖激酶对大肠杆菌NZN111产丁二酸的影响[J].生物工程学报,2011,27(10):1-10.
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备注/Memo

备注/Memo:
收稿日期:2011-09-22
基金项目:国家自然科学基金资助项目(21076105); 国家重点基础研究发展计划(973计划)资助项目(2009CB724701)
作者简介:刘嵘明(1985—),男,山东青岛人,博士研究生,研究方向:基因工程、发酵工程; 姜岷(联系人),教授,E-mail:bioengine@njut.edu.cn.
更新日期/Last Update: 2012-05-30