|本期目录/Table of Contents|

[1]连之娜,李鑫,欧阳嘉,等.2种纤维素酶水解效果比较及酶蛋白组分分析[J].生物加工过程,2010,8(06):57-62.[doi:10.3969/j.issn.1672-3678.2010.06.011]
 LIAN Zhi-na,LI Xin,OUYANG Jia,et al.Comparison between two kinds of cellulases in enzymatic hydrolysis and their secreted proteins analysis[J].Chinese Journal of Bioprocess Engineering,2010,8(06):57-62.[doi:10.3969/j.issn.1672-3678.2010.06.011]
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2种纤维素酶水解效果比较及酶蛋白组分分析(/HTML)
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
8
期数:
2010年06期
页码:
57-62
栏目:
出版日期:
2010-11-30

文章信息/Info

Title:
Comparison between two kinds of cellulases in enzymatic hydrolysis and their secreted proteins analysis
文章编号:
1672-3678(2010)06-0057-06
作者:
连之娜李鑫欧阳嘉陈牧勇强余世袁
南京林业大学林木遗传与生物技术省部共建教育部重点实验室,南京210037
Author(s):
LIAN Zhi-naLI XinOUYANG JiaCHEN MuYONG QiangYU Shi-yuan
Key Laboratory of Forest Genetics & Biotechnology of the Ministry of Education,Nanjing Forestry University,Nanjing 210037,China
关键词:
纤维素酶里氏木霉纤维素水解
分类号:
TQ351
DOI:
10.3969/j.issn.1672-3678.2010.06.011
文献标志码:
A
摘要:
比较了自产纤维素酶和商品纤维素酶的水解效果,并采用超滤、层析、SDS-PAGE相结合的方法分析2种纤维素酶蛋白组分的差异。里氏木霉以纸浆为C源合成的自产纤维素酶的水解得率高于商品纤维素酶,自产纤维素酶水解48 h的得率为66.24%,商品纤维素酶的得率为52.19%。自产纤维素酶中存在着Cel 6A酶组分和XYNⅡ酶组分,而商品纤维素酶中没有检测到这2种酶组分。自产纤维素酶和商品纤维素酶的Cel 1A酶组分和Cel 7A酶组分间存在着分布和含量上的差异。自产纤维素酶在相对分子质量(2.5~3.5)×104范围内存在着几条蛋白条带,而商品纤维素酶则是在相对分子质量3.5×104附近存在着几条蛋白条带。

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备注/Memo

备注/Memo:
收稿日期:2010-06-30
基金项目:国家重点基础研究发展计划(973计划)资助项目(2007CB707801);国家高技术研究发展计划(863计划)资助项目(2006AA020204)
作者简介:连之娜(1982—),女,山东威海人,硕士,实验员,研究方向:生物质资源的生物转化与利用,E-mail:lianzhina@yahoo.cn
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