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[1]韩立荣,祝传书,孙平平,等.青霉F10-2固态发酵植物农药残渣产纤维素酶[J].生物加工过程,2010,8(05):11-16.[doi:doi:10.3969/j.issn.1672-3678.2010.05.003]
 HAN Li-rong,ZHU Chuan-shu,SUN Ping-ping,et al.Solid state fermentation for producing cellulase by Penicillum waksmanii F10-2 by using plant pestcide residue[J].Chinese Journal of Bioprocess Engineering,2010,8(05):11-16.[doi:doi:10.3969/j.issn.1672-3678.2010.05.003]
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青霉F10-2固态发酵植物农药残渣产纤维素酶(/HTML)
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
8
期数:
2010年05期
页码:
11-16
栏目:
出版日期:
2010-09-30

文章信息/Info

Title:
Solid state fermentation for producing cellulase by Penicillum waksmanii F10-2 by using plant pestcide residue
文章编号:
1672-3678(2010)05-0011-06
作者:
韩立荣祝传书孙平平高保卫张兴
西北农林科技大学 无公害农药研究服务中心,陕西省生物农药工程技术研究中心,杨凌 712100
Author(s):
HAN Li-rongZHU Chuan-shuSUN Ping-ping GAO Bao-weiZHANG Xing
Research and Development Center of Biorational Pesticide,Technology and Engineering Center of Biopesticide,Northwest Agriculture & Forestry University,Yangling 712100, China
关键词:
纤维素酶瓦克青霉植物农药残渣固体发酵
分类号:
X712;TQ920.6
DOI:
doi:10.3969/j.issn.1672-3678.2010.05.003
文献标志码:
A
摘要:
采用单因素试验观察了不同C、N源、植物提取残渣与麦麸比例、初始pH和水料比对青霉F10-2菌株产纤维素酶的影响;在此基础上根据Box-wilson的中心组合试验设计原理,选取蛋白胨、初始pH和水料比等影响因素,采用三因素五水平的响应面分析方法,对瓦克青霉F10-2的固态产纤维素酶条件进行了优化。其优化后的产酶条件为m(川楝树皮残渣)∶m(麦麸)∶m(蛋白胨)∶m(KH2PO4)=80∶20∶1.4∶0.4、初始pH 6.2、水料比2∶1、26℃发酵8 d,在此条件下纤维素酶比酶活可达6.47 U/g,较原始培养条件提高了46.38%。

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备注/Memo

备注/Memo:
收稿日期:2009-11-18
基金项目:国家西部开发重大资助项目(2004BA901A14);陕西省“13115”资助项目(2007ZDGC-04)
作者简介:韩立荣(1979—),女,新疆塔城人,博士研究生,研究方向:微生物及农药学;张兴(联系人),教授,博士生导师,E-mail:zhxing1952@126.com
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