|本期目录/Table of Contents|

[1]朱启忠,杨万芹.壳聚糖载体的改性及其用于固定化漆酶的研究[J].生物加工过程,2010,8(04):33-38.[doi:1672-3678(2010)04-0033-06]
 ZHU Qizhong,YANG Wanqin.Modification of chitosan carrier for immobilization of laccase[J].Chinese Journal of Bioprocess Engineering,2010,8(04):33-38.[doi:1672-3678(2010)04-0033-06]
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壳聚糖载体的改性及其用于固定化漆酶的研究(/HTML)
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
8
期数:
2010年04期
页码:
33-38
栏目:
出版日期:
2010-07-31

文章信息/Info

Title:
Modification of chitosan carrier for immobilization of laccase
作者:
朱启忠杨万芹
山东大学威海分校海洋学院,威海264209
Author(s):
ZHU QizhongYANG Wanqin
College of Oceanology, Weihai Campus of Shandong University,Weihai 264209,China
关键词:
壳聚糖固定化漆酶有机酸
分类号:
Q814.2
DOI:
1672-3678(2010)04-0033-06
文献标志码:
A
摘要:
利用有机酸改性壳聚糖,并用交联法制备酸化的壳聚糖载体,然后用改性壳聚糖载体固定漆酶.甲酸、乙酸改性壳聚糖的最适条件:壳聚糖与甲酸、乙酸的质量摩尔比(g/mol)分别为100:1、100:1.5,戊二醛的质量分数为1%,缓冲溶液的pH分别是4.4、5.0,反应时间为3 h;壳聚糖与酒石酸、草酸的质量摩尔比(g/mol)分别为100:0.5、100:2,戊二醛的质量分数为2%,缓冲溶液的pH分别是3.6、4.2,反应时间为4.5 h.不同有机酸改性的壳聚糖用于漆酶的固定,其酶活都有不同程度的提高,尤其用酒石酸改性的壳聚糖载体效果最好,其酶活提高了57%.

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