|本期目录/Table of Contents|

[1]宋娜娜,宋向阳,欧阳嘉,等.里氏木霉与黑曲霉混合发酵产纤维素酶及其水解特性[J].生物加工过程,2010,8(05):5-10.[doi:doi:10.3969/j.issn.1672-3678.2010.05.002]
 SONG Na-na,SONG Xiang-yang,OUYANG Jia,et al.Cellulose production from fungal mixed culture of Trichoderma reesei RUT C-30 and Apsergillus niger NL02 on steam-exploded corn stover and characteristics of hydrolysis[J].Chinese Journal of Bioprocess Engineering,2010,8(05):5-10.[doi:doi:10.3969/j.issn.1672-3678.2010.05.002]
点击复制

里氏木霉与黑曲霉混合发酵产纤维素酶及其水解特性(/HTML)
分享到:

《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
8
期数:
2010年05期
页码:
5-10
栏目:
出版日期:
2010-09-30

文章信息/Info

Title:
Cellulose production from fungal mixed culture of Trichoderma reesei RUT C-30 and Apsergillus niger NL02 on steam-exploded corn stover and characteristics of hydrolysis
文章编号:
1672-3678(2010)05-0005-06
作者:
宋娜娜宋向阳欧阳嘉勇强
南京林业大学化学工程学院,林木遗传与生物技术省部共建教育部重点实验室,南京 210037
Author(s):
SONG Na-naSONG Xiang-yangOUYANG JiaYONG Qiang
College of Chemical Engineering, Key Laboratory of Forest Genetics and Biotechnology,Ministry of Education,Nanjing Forestry Universtiy, Nanjing 210037,China
关键词:
黑曲霉纤维素酶玉米秸秆水解混合培养里氏木霉
分类号:
TQ351
DOI:
doi:10.3969/j.issn.1672-3678.2010.05.002
文献标志码:
A
摘要:
研究了利用里氏木霉和黑曲霉混合培养产纤维素酶,以黑曲霉孢子悬浮液的不同活化浓度及不同的活化时间来寻找2个菌种发挥最大协同作用的结合点以及所产纤维素酶的水解特性。 以里氏木霉单一培养和黑曲霉单一培养为参照进行对比研究。底物为农林废弃物之一的玉米秸秆,经过蒸气爆破预处理后,用作产酶C源。 结果表明:黑曲霉孢子悬浮液活化浓度为10个/mL,活化时间为12 h,滤纸酶比酶活最高,达3.32 U/mL,高于里氏木霉单一培养的2.25 U/mL,β-葡萄糖苷酶比酶活达1.32 U/mL,高于里氏木霉单一培养的0.57 U/mL。为进一步验证混合菌产纤维素酶的水解效果,利用混合菌产纤维酶的酶液及里氏木霉产纤维素酶的酶液进行酶水解实验,当酶用量为20 U/g绝干纤维素,底物质量浓度为100 g/L条件下水解48 h,混合菌所产酶液酶解得率达70.00%,高于里氏木霉所产酶液的酶解得率63.05%。实验表明里氏木霉与黑曲霉混合培养产酶是可行的&并优于单一菌种培养。

相似文献/References:

[1]韩立荣,祝传书,孙平平,等.青霉F10-2固态发酵植物农药残渣产纤维素酶[J].生物加工过程,2010,8(05):11.[doi:doi:10.3969/j.issn.1672-3678.2010.05.003]
 HAN Li-rong,ZHU Chuan-shu,SUN Ping-ping,et al.Solid state fermentation for producing cellulase by Penicillum waksmanii F10-2 by using plant pestcide residue[J].Chinese Journal of Bioprocess Engineering,2010,8(05):11.[doi:doi:10.3969/j.issn.1672-3678.2010.05.003]
[2]连之娜,李鑫,欧阳嘉,等.2种纤维素酶水解效果比较及酶蛋白组分分析[J].生物加工过程,2010,8(06):57.[doi:10.3969/j.issn.1672-3678.2010.06.011]
 LIAN Zhi-na,LI Xin,OUYANG Jia,et al.Comparison between two kinds of cellulases in enzymatic hydrolysis and their secreted proteins analysis[J].Chinese Journal of Bioprocess Engineering,2010,8(05):57.[doi:10.3969/j.issn.1672-3678.2010.06.011]
[3]于莹,姚日生,齐本坤,等.绿色木霉ZY-1固态发酵产纤维素酶[J].生物加工过程,2009,7(01):54.
 YU Ying,YAO Ri-sheng,QI Ben-kun,et al.Cellulase production by Trichoderma viride ZY-1 under solid-state fermentation[J].Chinese Journal of Bioprocess Engineering,2009,7(05):54.
[4]许云峰,周建芹,陈晶磊,等.内生青霉菌纤维素酶辅助提取槐米总黄酮[J].生物加工过程,2009,7(02):18.
 XU Yun-feng,ZHOU Jian-qin,CHEN Jing-lei,et al.Application of cellulase from endophytic Penicillium sp.B-4 on total flavonoid extraction from flower buds of Sophara japonica[J].Chinese Journal of Bioprocess Engineering,2009,7(05):18.
[5]乐易林,邵蔚蓝.极端耐热纤维素酶Cel74的表达、纯化与特性[J].生物加工过程,2009,7(03):64.
 LE Yi-lin,SHAO Wei-lan,Expression and characterization of Thermotoga maritima cellulase Cel74[J].Chinese Journal of Bioprocess Engineering,2009,7(05):64.
[6]朱年青,夏文静,勇强.里氏木霉纤维素酶的分离纯化及酶学性质[J].生物加工过程,2010,8(03):40.[doi:10.3969/j.issn.1672-3678.2010.03.008]
 ZHU Nian-qing,XIA Wen-jing,YONG Qiang.Purification and characterization of cellulase from Trichoderma reesei[J].Chinese Journal of Bioprocess Engineering,2010,8(05):40.[doi:10.3969/j.issn.1672-3678.2010.03.008]
[7]梁娟然,戴菲辉,苏思莹,等.纤维素酶在二甲基亚砜微扰条件下的动力学行为和光谱学变化[J].生物加工过程,2011,9(02):29.[doi:doi:10.3969/j.issn.1672-3678.2011.02.006]
 LIANG Juanran,DAI Feihui,SU Siying,et al.Kinetics and spectrum changes of cellulase in dimethyl sulfoxide solution[J].Chinese Journal of Bioprocess Engineering,2011,9(05):29.[doi:doi:10.3969/j.issn.1672-3678.2011.02.006]
[8]胡慧东,许赣荣.黑曲霉固态发酵及酶解玉米皮[J].生物加工过程,2011,9(02):39.[doi:doi:10.3969/j.issn.1672-3678.2011.02.008]
 HU Huidong,XU Ganrong.Degradation of corn bran by solid-state fermentation using Aspergillus niger[J].Chinese Journal of Bioprocess Engineering,2011,9(05):39.[doi:doi:10.3969/j.issn.1672-3678.2011.02.008]
[9]祖彩霞,李志敏,叶勤.里氏木霉Trichoderma reesei产纤维素酶的发酵培养基碳氮源优化[J].生物加工过程,2011,9(04):6.[doi:10.3969/j.issn.1672-3678.2011.04.002]
 ZU Caixia,LI Zhimin,YE Qin.Culture medium optimization for producing cellulase by Trichoderma reesei[J].Chinese Journal of Bioprocess Engineering,2011,9(05):6.[doi:10.3969/j.issn.1672-3678.2011.04.002]
[10]李淑丽,王娇,尹清强,等.木聚糖酶产生菌的筛选与酶学生化特性[J].生物加工过程,2012,10(04):54.[doi:10.3969/j.issn.1672-3678.2012.04.012]
 LI Shuli,WANG Jiao,YIN Qingqiang,et al.Isolation of xylanase-producing microbes and analysis of its biochemical properties[J].Chinese Journal of Bioprocess Engineering,2012,10(05):54.[doi:10.3969/j.issn.1672-3678.2012.04.012]

备注/Memo

备注/Memo:
收稿日期:2010-04-21
基金项目:国家自然科学基金资助项目(30871992);国家高技术研究发展计划(863 计划)资助项目(2008AA05Z401)
作者简介:宋娜娜(1985—),女,江苏南京人,硕士研究生,研究方向:生物燃料乙醇;宋向阳(联系人),副教授,E-mail:xiangyangsong@hotmail.com
更新日期/Last Update: