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[1]孙鹏,张文,倪晔,等.羰基还原酶产生菌 SW 2026的产酶条件及其不对称催化还原4′-氯苯乙酮[J].生物加工过程,2009,7(05):19-24.
 SUN Peng,ZHANG Wen,NI Ye,et al.Enhancement of asymmetric reduction of 4′-chloroacetophenone by optimizing culture conditions of carbonyl reductase-producing strain Candida krusei SW 2026 [J].Chinese Journal of Bioprocess Engineering,2009,7(05):19-24.
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羰基还原酶产生菌 SW 2026的产酶条件及其不对称催化还原4′-氯苯乙酮
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
7
期数:
2009年05期
页码:
19-24
栏目:
出版日期:
2009-09-30

文章信息/Info

Title:
Enhancement of asymmetric reduction of 4′-chloroacetophenone by optimizing culture conditions of carbonyl reductase-producing strain Candida krusei SW 2026


文章编号:
1672-3678(2009)05-0019-06
作者:
孙鹏张文倪晔郑璞孙志浩
江南大学 生物工程学院 工业生物技术教育部重点实验室, 无锡214122
Author(s):
SUN PengZHANG WenNI Ye ZHENG PuSUN Zhi-hao
Key Laboratory of Industrial Biotechnology of the Ministry of Education,School of Biotechnology, Jiangnan University, Wuxi 214122, China
关键词:
不对称还原4′-氯苯乙酮芳香酮产酶条件
分类号:
Q554+.9
文献标志码:
A
摘要:
以4′-氯苯乙酮为模型底物,对筛选得到的Candida krusei SW 2026的羰基还原酶产酶条件进行研究。结果表明:适宜的发酵培养基组成为甘油50 g/L,玉米浆20 g/L,KH2PO4 4 g/L,MgSO4·7H2O 1.5 g/L;适宜的培养条件为温度30 ℃,初始pH 6,摇床转速200 r/min,发酵周期 48 h。在产酶发酵条件下培养的湿细胞对4′-氯苯乙酮进行不对称还原反应,产物(S)-4′-氯-α-苯乙醇的产率最高达88.56%,e.e.值稳定在87%左右。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2008-12-30
基金项目:国家高技术研究发展计划(863计划)资助项目(2007AA02Z226)
作者简介:孙鹏(1984—),男,湖北老河口人,硕士研究生,研究方向:生物催化;倪晔(联系人),副教授, E-mail:yni@jiangnan.edu.cn
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