|本期目录/Table of Contents|

[1]乐易林,邵蔚蓝.极端耐热纤维素酶Cel74的表达、纯化与特性[J].生物加工过程,2009,7(03):64-67.
 LE Yi-lin,SHAO Wei-lan,Expression and characterization of Thermotoga maritima cellulase Cel74[J].Chinese Journal of Bioprocess Engineering,2009,7(03):64-67.
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极端耐热纤维素酶Cel74的表达、纯化与特性
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
7
期数:
2009年03期
页码:
64-67
栏目:
出版日期:
2009-05-30

文章信息/Info

Title:
Expression and characterization of Thermotoga maritima cellulase Cel74
文章编号:
1672-3678(2009)03-0064-04
作者:
乐易林1邵蔚蓝12
1. 江南大学 生物工程学院,无锡214122;
2. 南京师范大学 生命科学学院,南京210046
Author(s):
LE Yi-lin1 SHAO Wei-lan1 2
1. School of Biotechnology, Jiangnan University, Wuxi 214122, China;
2. Department of Life Science, Nanjing Normal University, Nanjing 210046, China
关键词:
海栖热袍菌纤维素酶耐热性
分类号:
Q814.1
文献标志码:
A
摘要:
从海栖热袍菌中克隆出编码热稳定性的纤维素酶基因,以热激载体pHsh为表达质粒,构建重组质粒phsh-Cel74,并转化至大肠杆菌中进行表达。基因表达产物通过热处理和离子交换层析,重组酶纯度达电泳纯。对纯化的重组酶酶学性质研究表明,最适反应温度85 ℃,最适反应pH 4.6,pH 4.5~6.0之间酶的相对酶活在80%以上。Co2+ 对酶活性有促进作用,Ca2+、Mg2+、Zn2+ 不影响酶活性,而Cu2+、Ni2+、Mn2+ 对酶活性有抑制作用。

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备注/Memo

备注/Memo:
收稿日期:2008-10-21
作者简介:乐易林(1978—),男,江西宜春人,博士研究生,研究方向:基因工程;邵蔚蓝(联系人),教授,E-mail: wlshao@jsmail.com.cn
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