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[1]许云峰,周建芹,陈晶磊,等.内生青霉菌纤维素酶辅助提取槐米总黄酮[J].生物加工过程,2009,7(02):18-22.
 XU Yun-feng,ZHOU Jian-qin,CHEN Jing-lei,et al.Application of cellulase from endophytic Penicillium sp.B-4 on total flavonoid extraction from flower buds of Sophara japonica[J].Chinese Journal of Bioprocess Engineering,2009,7(02):18-22.
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内生青霉菌纤维素酶辅助提取槐米总黄酮
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
7
期数:
2009年02期
页码:
18-22
栏目:
出版日期:
2009-03-30

文章信息/Info

Title:
Application of cellulase from endophytic Penicillium sp.B-4 on total flavonoid extraction from flower buds of Sophara japonica
文章编号:
1672-3678(2009)02-0018-05
作者:
许云峰周建芹陈晶磊王剑文
苏州大学 药学院,苏州 215123
Author(s):
XU Yun-fengZHOU Jian-qinCHEN Jing-leiWANG Jian-wen
School of Pharmaceutical Sciences,Soochow University,Suzhou 215123,China
关键词:
纤维素酶内生提取青霉菌总黄酮
分类号:
R284.2
文献标志码:
A
摘要:
研究内生青霉菌(Penicillium sp.B4)胞外纤维素酶在槐米总黄酮提取中的辅助应用。内生青霉菌在起始pH 4.5的综合马铃薯培养基中,150 r/min,40 ℃下摇瓶,培养7 d,具有较高的纤维素酶比活力(3.57 U/mL)。槐米干粉投入青霉菌发酵液中进行酶解处理,比较酶料比、酶解温度、酶解时间和酶解液pH对槐米总黄酮提取率的影响,发现槐米干粉以酶料比40∶1(mL/g)加入粗酶液中,在pH 4.5、温度40 ℃下酶解处理1 h后,黄酮提取率可达12.2%,比常规提取率增加了38.7%。内生菌纤维素酶辅助提取法为槐米黄酮提取的可行新方法。

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备注/Memo

备注/Memo:
收稿日期:2008-06-23
基金项目:国家自然科学基金资助项目(30772731);江苏省自然科学基金资助项目(BK2007051);苏州大学青年教师研究基金资助项目(Q3132625)
作者简介:许云峰(1983—),男,江苏盐城人,硕士研究生,研究方向:中药生物技术;王剑文(联系人),研究员,E-mail:jwwang@suda.edu.cn
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