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[1]于莹,姚日生,齐本坤,等.绿色木霉ZY-1固态发酵产纤维素酶[J].生物加工过程,2009,7(01):54-58.
 YU Ying,YAO Ri-sheng,QI Ben-kun,et al.Cellulase production by Trichoderma viride ZY-1 under solid-state fermentation[J].Chinese Journal of Bioprocess Engineering,2009,7(01):54-58.
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绿色木霉ZY-1固态发酵产纤维素酶
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
7
期数:
2009年01期
页码:
54-58
栏目:
出版日期:
2009-01-30

文章信息/Info

Title:
Cellulase production by Trichoderma viride ZY-1 under solid-state fermentation
文章编号:
1672-3678(2009)01-0054-05
作者:
于莹1姚日生12齐本坤1朱慧霞12
1. 合肥工业大学 化学工程学院,合肥? 230009;
2. 合肥工业大学 农产品生物化工教育部工程研究中心,合肥 230009
Author(s):
YU Ying1 YAO Ri-sheng12 QI Ben-kun1 ZHU Hui-xia12
1. School of Chemical Engineering, Hefei University of Technology, Hefei 230009, China;
2. Engineering Research Center of Bioprocess of Ministry of Education, Hefei University of Technology,Hefei 230009, China
关键词:
纤维素酶酶活稻草固态发酵麸皮
分类号:
Q539.3
文献标志码:
A
摘要:
利用筛选的绿色木霉ZY-1(Trichoderma viride ZY-1)固态发酵产纤维素酶,采用稻草和麸皮为底物,考察稻草与麸皮比例随发酵时间对产酶的影响。结果表明:底物中,在m(稻草)∶m(麸皮)为0∶5和1∶4时,发酵48 h,pH保持4.5左右,还原糖量急剧上升,胞外蛋白产量最低;仅以稻草作底物时,整个发酵过程中pH约为7,还原糖量最低,胞外蛋白产量较高而滤纸酶活、羧甲基纤维素酶(CMCase)和β-葡萄糖苷酶(β-Gase)酶活均较低;在m(稻草)∶m(麸皮)为3∶2时,发酵96 h,滤纸酶活达最大值5.01 U/g干曲;m(稻草):m(麸皮)为1∶4时,发酵96 h,β-Gase酶活达最大值4.6 U/g干曲;m(稻草):m(麸皮)为4∶1时,发酵72 h,CMCase酶活达最大值6.01 U/g干曲。因此,底物中存在适量的稻草和麸皮有利于Trichoderma viride ZY-1产纤维素酶。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2008-05-21
基金项目:合肥工业大学科学研究发展基金资助项目(060604F)
作者简介:于莹(1983—),女,河南开封人,硕士研究生, 研究方向:微生物发酵及酶工程;姚日生(联系人),教授,E-mail:yaors@163.com
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