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[1]孟晨璐,张梁,丁重阳,等.构建羰基还原酶基因工程菌生物转化产l-麻黄碱[J].生物加工过程,2009,7(01):29-33.[doi:006]
 MENG Chen-lu,ZHANG Liang,DING Chong-yang,et al.Construction and application of carbonyl reductase gene engineering strain in biosynthesis of l-ephedrine[J].Chinese Journal of Bioprocess Engineering,2009,7(01):29-33.[doi:006]
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构建羰基还原酶基因工程菌生物转化产l-麻黄碱
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
7
期数:
2009年01期
页码:
29-33
栏目:
出版日期:
2009-01-30

文章信息/Info

Title:
Construction and application of carbonyl reductase gene engineering strain in biosynthesis of l-ephedrine
文章编号:
1672-3678(2009)01-0029-05
作者:
孟晨璐 张梁丁重阳 王正祥石贵阳
江南大学 生物工程学院,无锡214122
Author(s):
MENG Chen-lu ZHANG Liang DING Chong-yang WANG Zheng-xiang SHI Gui-yang
School of Biotechnology, Jiangnan University, Wuxi 214122, China
关键词:
不对称还原羰基还原酶麻黄碱
分类号:
Q789; TQ920.6
DOI:
006
文献标志码:
A
摘要:
以筛选得到的Morganella morganii J-8细菌的基因组为模板,通过PCR扩增得到目的基因mdlh2。核苷酸序列测定结果表明,基因全长1 046 bp。以pET28a(+)为表达载体,构建重组质粒pET28a(+)-mldh2,并在E.coli BL21(DE3)中表达。利用表达产物进行生物转化,发现其具有催化底物1-苯基-2-甲氨基丙酮(简称MAK)产l-麻黄碱的活力。进一步考察了诱导时间和IPTG浓度对重组菌表达的羰基还原酶的影响,37 ℃下用0.5 mmol/L的IPTG诱导4 h,重组羰基还原酶的酶活达到0.2 U/mg蛋白,转化液中l-麻黄碱质量浓度达到45 mg/L。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2008-04-25
基金项目:国家自然科学基金资助项目(30770054);国家高技术研究发展计划(863计划)资助项目(2007AA100402)
作者简介:孟晨璐(1983—),女,辽宁鞍山人,硕士研究生,研究方向:生物资源与生物转化;石贵阳(联系人),教授,博士生导师,E-mail:gyshi@jiangnan.edu.cn
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