|本期目录/Table of Contents|

[1]郑晓冰,郭丽琼,付小燕,等.木霉LaTr 01菌株产漆酶发酵的条件[J].生物加工过程,2007,5(04):19-24.[doi:10.3969/j.issn.1672-3678.2007.04.005]
 ZHENG Xiao-bing,GUO Li-qiong,FU Xiao-yan,et al.Optimization of laccase production conditions of a newly isolated Trichoderma spp. strain LaTr 01 from southern China[J].Chinese Journal of Bioprocess Engineering,2007,5(04):19-24.[doi:10.3969/j.issn.1672-3678.2007.04.005]
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木霉LaTr 01菌株产漆酶发酵的条件()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
5
期数:
2007年04期
页码:
19-24
栏目:
出版日期:
2007-11-30

文章信息/Info

Title:
Optimization of laccase production conditions of a newly isolated Trichoderma spp. strain LaTr 01 from southern China
作者:
郑晓冰1郭丽琼2付小燕1刁惠萍1林俊芳2
1. 华南农业大学, 食品学院生物工程系, 广州, 510640; 2. 华南农业大学, 食品学院生物工程系, 广州, 510640; 华南农业大学, 生物质能研究所, 广州, 510640
Author(s):
ZHENG Xiao-bing1 GUO Li-qiong2 FU Xiao-yan1 DIAO Hui-ping1 LIN Jun-fang2
关键词:
木霉漆酶漆酶产酶条件正交试验
分类号:
Q554
DOI:
10.3969/j.issn.1672-3678.2007.04.005
摘要:
从华南地区采集土样,采用愈创木酚平板筛选产漆酶菌株,获得了一株短周期产漆酶的小型丝状真菌.通过观察菌落特征、生长情况以及显微镜下菌丝和孢子的形态,初步鉴定该菌株为木霉属的一个种(Trichoderma spp.),命名为木霉LaTr 01菌株.通过单因素方法研究该菌产漆酶的发酵条件,结果表明:LaTr 01的产酶培养基以麦芽糖为最佳碳源;以酵母提取物为最适氮源;培养24 h后加入Cu2+比培养开始加入Cu2+ LaTr 01产酶活高出约1倍.采用麦芽糖、酵母提取物、Cu2+浓度L9(33)的正交试验优化漆酶发酵条件,结果表明,氮源是影响该菌产漆酶的最重要因素,碳源次之,Cu2+浓度影响较小;LaTr 01菌株产生漆酶的最佳条件为:5 g/L酵母提取物、20 g/L麦芽糖、1.5 mmol/L Cu2+,Cu2+加入时间为培养24 h后.在优化的培养条件下,该菌酶活可达480.556 U/L.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金,广东省科技攻关计划,广东省自然科学基金
更新日期/Last Update: 1900-01-01