|本期目录/Table of Contents|

[1]李永建,严明,丁莉,等.在酿酒酵母中共表达XYLA和XKS1基因后利用木糖的初步研究[J].生物加工过程,2006,4(04):65-69.[doi:10.3969/j.issn.1672-3678.2006.04.014]
 LI Yong-jian,YAN Ming,DING Li,et al.Construction of Saccharomyces cerevisiae expressing XYLA and XKS1 and study on the effect of the xylose utilization[J].Chinese Journal of Bioprocess Engineering,2006,4(04):65-69.[doi:10.3969/j.issn.1672-3678.2006.04.014]
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在酿酒酵母中共表达XYLA和XKS1基因后利用木糖的初步研究()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
4
期数:
2006年04期
页码:
65-69
栏目:
出版日期:
2006-11-30

文章信息/Info

Title:
Construction of Saccharomyces cerevisiae expressing XYLA and XKS1 and study on the effect of the xylose utilization
作者:
李永建严明丁莉许伟许琳
南京工业大学, 制药与生命科学学院, 南京, 210009
Author(s):
LI Yong-jian YAN Ming DING Li XU Wei XU Lin
关键词:
木糖异构酶木酮糖激酶木糖酿酒酵母乙醇
分类号:
Q786
DOI:
10.3969/j.issn.1672-3678.2006.04.014
摘要:
为了使酿酒酵母较好地利用木糖产生乙醇,将来自Thermus thermophilus的木糖异构酶基因XYLA和酿酒酵母自身的木酮糖激酶基因XKS1,构建到酵母表达载体pESC-LEU中,导入酿酒酵母YPH499中,同时成功表达了两种酶基因.该菌以木糖为唯一碳源进行限氧发酵,木糖的利用率为9.64%,为宿主菌的4.17倍,产生2.22 mmol·L-1的乙醇.同时初步探讨了两种酶基因的表达量对酿酒酵母发酵木糖生成乙醇的影响.木糖异构酶对木糖的利用起关键性的作用,木酮糖激酶的过量表达不利于乙醇生成.

参考文献/References:

[1] Anil Lachke. Biofuel from D-xylose the second most abundant sugar [J]. Resource, 2002.50-58.doi:10.1007/BF02836736.
[2] Walfridsson M, Anderlund M, Bao X. Expression of different levels of enzymes from the Pichia stipitis XYL1 and XYL2 genes in Saccharomyces cerevisiae and its effects on product formation during xylose utilisation [J]. Applied Microbiology and Biotechnology, 1997(2):218-224.doi:10.1007/s002530051041.
[3] Walfridsson M, Bao X, Anderlund M. Ethanolic fermentation of xylose with Saccharomyces cerevisiae harboring the Thermus thermophilus xylA gene which expresses an active xylose (glucose) isomerase [J]. Applied and Environmental Microbiology, 1996, (12):4648-4651.
[4] 沈煜, 王颖, 史文龙. 酿酒酵母工业菌株中XI木糖代谢途径的建立 [J]. 中国生物工程杂志, 2005(9):69-73.doi:10.3969/j.issn.1671-8135.2005.09.015.
[5] Anna E, Camilla C, Fredrik W. Anaerobic xylose fermentation by recombinant Saccharomyces cerevisiae carrying XYL1, XYL2, and XKS1 in mineral medium chemostat cultures [J]. Applied and Environmental Microbiology, 2000(8):3381-3386.doi:10.1128/AEM.66.8.3381-3386.2000.
[6] Toivari M H, Aristidou A, Ruohonen L. Conversion of xylose to ethanol by recombinant Saccharomyces cerevisiae:importance of xylulokinase (XKS1) and oxygen availability [J]. Metabolic Engineering, 2001(3):236-249.doi:10.1006/mben.2000.0191.
[7] 刘小琳, 江宁, 贺鹏. 酿酒酵母菌共表达XYLA和XKS1发酵木糖生产酒精 [J]. 科学通报, 2005(7):653-658.doi:10.3321/j.issn:0023-074X.2005.07.008.
[8] 萨姆布鲁克·J, 弗里奇E·F, 曼尼阿蒂斯 T, 金冬雁 黎孟枫. 分子克隆实验指南 [M]. 北京:科学出版社, 2002.
[9] 许伟, 严明, 李永建. Ecoli 木糖异构酶基因的克隆及表达条件的优化 [J]. 生物加工过程, 2005(4):45-48.doi:10.3969/j.issn.1672-3678.2005.04.010.
[10] Adams A, Gottschling D E, Kaiser C A. Methods in yeasts genetics:cold spring harbor laboratory course manual [M]. New York:Cold Spring Harbor Laboratory Press, 1997.
[11] Dische Z, Borenfreund E. A new spectrophotometric method for the detection and determination of keto sugars and trioses [J]. Journal of Biological Chemistry, 1951.583-587.
[12] Flanagan T, Waites M J. Purification and characterization of D-xylulokinase from the pentose-fermenting yeast Pichia stipitisNCYC-1541 [J]. Enzyme and Microbial Technology, 1992.975-979.doi:10.1016/0141-0229(92)90080-8.
[13] 沈煜, 王颖, 鲍晓明. 酿酒酵母木糖发酵酒精途径工程的研究进展 [J]. 生物工程学报, 2004(5):636-640.doi:10.3321/j.issn:1000-3061.2003.05.025.
[14] Lai K, Elsas L J. Overexpression of human UDP-glucose pyrophosphorylase rescues galactose-1-phosphate uridyltransferase-deficient yeast [J]. Biochemical and Biophysical Research Communications, 2000(2):392-400.doi:10.1006/bbrc.2000.2629.
[15] Jin Y S, Ni H Y. Optimal growth and ethanol production from xylose by recombinant Saccharomyces cerevisiae require moderate D-Xylulokinase activity [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003(1):495-530.
[16] Anna E, Camilla C, Fredrik W. Anaerobic xylosefermentation by recombinant S accharomyces cerevisiae carryingXYL1, XYL2, and XKS1 in mineral medium chemostatcultures [J]. Applied and Environmental Microbiology, 2000(8):3381-3386.doi:10.1128/AEM.66.8.3381-3386.2000.

相似文献/References:

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[2]侯进,沈煜,鲍晓明.木糖异构酶在酿酒酵母表面表达及对木糖代谢影响的初步研究[J].生物加工过程,2006,4(01):30.[doi:10.3969/j.issn.1672-3678.2006.01.007]
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备注/Memo

备注/Memo:
基金项目:国家重点基础研究发展计划(973计划),国家自然科学基金
更新日期/Last Update: 1900-01-01