|本期目录/Table of Contents|

[1]叶逢春,陈银,邢新会.重组大肠杆菌生产可溶性MBP融合肝素酶的培养条件优化[J].生物加工过程,2006,4(03):28-32.[doi:10.3969/j.issn.1672-3678.2006.03.005]
 YE Feng-chun,CHEN Yin,XING Xin-hui.Optimization of cultivation conditions of recombinant E. coli for production of soluble MBP-fused heparinase Ⅰ[J].Chinese Journal of Bioprocess Engineering,2006,4(03):28-32.[doi:10.3969/j.issn.1672-3678.2006.03.005]
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重组大肠杆菌生产可溶性MBP融合肝素酶的培养条件优化()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
4
期数:
2006年03期
页码:
28-32
栏目:
出版日期:
2006-08-30

文章信息/Info

Title:
Optimization of cultivation conditions of recombinant E. coli for production of soluble MBP-fused heparinase Ⅰ
作者:
叶逢春陈银邢新会
清华大学, 化学工程系生物化工研究所, 北京, 100084
Author(s):
YE Feng-chun CHEN Yin XING Xin-hui
关键词:
肝素酶大肠杆菌麦芽糖结合蛋白优化可溶性表达
分类号:
TQ920.1
DOI:
10.3969/j.issn.1672-3678.2006.03.005
摘要:
为确立肝素酶Ⅰ的高效生产工艺,利用麦芽糖结合蛋白(MBP)与肝素酶Ⅰ融合性能,通过构建相应的表达质粒pMHS,在大肠杆菌方面实现了肝素酶Ⅰ可溶性表达.通过对LB培养基摇瓶培养E.coli TB1(pMHS)的诱导时机、诱导剂用量以及添加葡萄糖、酵母提取物、乙醇、氯霉素和卡那霉素等一系列培养条件的优化,确定了该可溶性肝素酶融合蛋白MBP-hepA的最佳生产条件.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金
更新日期/Last Update: 1900-01-01