|本期目录/Table of Contents|

[1]胡健,徐岩.不对称还原2-辛酮的菌株筛选和反应条件的研究[J].生物加工过程,2005,3(03):42-46.[doi:10.3969/j.issn.1672-3678.2005.03.009]
 HU Jian,XU Yan.Screening and optimization of asymmetric reduction of 2-octanone by Oenococcus oeni CECT 4730[J].Chinese Journal of Bioprocess Engineering,2005,3(03):42-46.[doi:10.3969/j.issn.1672-3678.2005.03.009]
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不对称还原2-辛酮的菌株筛选和反应条件的研究()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
3
期数:
2005年03期
页码:
42-46
栏目:
出版日期:
2005-08-30

文章信息/Info

Title:
Screening and optimization of asymmetric reduction of 2-octanone by Oenococcus oeni CECT 4730
作者:
胡健徐岩
江南大学, 生物工程学院教育部工业生物技术重点实验室, 无锡, 214036
Author(s):
HU Jian XU Yan
关键词:
不对称还原2-辛醇菌株筛选全细胞生物催化两相反应
分类号:
TQ033
DOI:
10.3969/j.issn.1672-3678.2005.03.009
摘要:
从本实验室保藏菌株中筛选出一株酒明串珠菌Oenococcus oeni CECT 4730,能不对称还原2-辛酮得到R-2-辛醇.对反应条件的研究表明:Tris-硼酸(TBE)是最佳缓冲液,葡萄糖是最佳辅助底物,壬烷是最佳有机相,当采用300 mmol/L的TBE缓冲液、300 mmol/L的葡萄糖、1:1(体积比)的两相比、150 g/L的湿菌体质量浓度下,可以实现3%的2-辛酮转化为R-2-辛醇(转化率>99%),并且e.e.值为97%.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:中国科学院资助项目
更新日期/Last Update: 1900-01-01