|本期目录/Table of Contents|

[1]吕乾川,伍元东,周俊,等.一株柴油高级烃降解菌的筛选与鉴定[J].生物加工过程,2017,15(04):57-63.[doi:10.3969/j.issn.1672-3678.2017.04.010]
 LYU Qianchuan,WU Yuandong,ZHOU Jun,et al.Isolation and identification of a diesel alkanes degrading bacterium[J].Chinese Journal of Bioprocess Engineering,2017,15(04):57-63.[doi:10.3969/j.issn.1672-3678.2017.04.010]
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一株柴油高级烃降解菌的筛选与鉴定()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
15
期数:
2017年04期
页码:
57-63
栏目:
出版日期:
2017-07-30

文章信息/Info

Title:
Isolation and identification of a diesel alkanes degrading bacterium
文章编号:
1672-3678(2017)04-0057-07
作者:
吕乾川12伍元东12周俊12贾红华12韦萍1郑涛3雍晓雨12
1.南京工业大学 生物与制药工程学院,江苏 南京 211800; 2.南京工业大学 生物能源研究所,江苏 南京 211800; 3.中国科学院 广州能源研究所,广东 广州 510640
Author(s):
LYU Qianchuan12WU Yuandong12ZHOU Jun12JIA Honghua12WEI Ping1ZHENG Tao3YONG Xiaoyu12
1.College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211800,China; 2.Bioenergy Research Institute,Nanjing Tech University,Nanjing 211800,China; 3.Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences,Guangzhou 510640,China
关键词:
柴油高级烃 直链烷烃 红平红球菌 降解菌
分类号:
Q945.78
DOI:
10.3969/j.issn.1672-3678.2017.04.010
文献标志码:
A
摘要:
从加油站附近土壤取样,以不同的柴油高级烃为碳源、菌体生物量(OD600)为指示,分离筛选柴油高级烃降解菌株,同时应用高效液相色谱(HPLC)、气相色谱-质谱(GC-MS)分析其降解率以及降解产物。结果筛选到一株对二苯并噻吩、芳香烃类都具有一定降解效果的菌株B5,它对二苯并噻吩的降解率为63.0%,芳香烃类降解率为83.4%。该菌株能将直链烷烃(如正十二烷)的末端羧基化形成羧酸类物质,在实际应用中具有提高油品润滑性质的潜力。经16S rRNA基因鉴定,该菌株为一株红平红球菌(Rhodococcus erythropolis),并被命名为R.erythropolisB5。

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备注/Memo

备注/Memo:
收稿日期:2017-03-23
基金项目:国家重点基础研究发展计划(973计划)(2013CB733904); 江苏省自然科学基金(BK20130932)
作者简介:吕乾川(1989—),男,江苏南京人,研究方向:生物化工; 雍晓雨(联系人),讲师,E-mail:yongxiaoyu@njtech.edu.cn.
更新日期/Last Update: 2017-07-30