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[1]郭超林,丁重阳,陆健,等.阿魏蘑与胶红酵母共培养中促进产漆酶物质的初步研究[J].生物加工过程,2017,15(04):40-44.[doi:10.3969/j.issn.1672-3678.2017.04.007]
 GUO Chaolin,DING Zhongyang,LU Jian,et al.Compound to enhance laccase production in co-culture of Pleurotus ferulae and Rhodotorula mucilaginosa[J].Chinese Journal of Bioprocess Engineering,2017,15(04):40-44.[doi:10.3969/j.issn.1672-3678.2017.04.007]
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阿魏蘑与胶红酵母共培养中促进产漆酶物质的初步研究()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
15
期数:
2017年04期
页码:
40-44
栏目:
出版日期:
2017-07-30

文章信息/Info

Title:
Compound to enhance laccase production in co-culture of Pleurotus ferulae and Rhodotorula mucilaginosa
文章编号:
1672-3678(2017)04-0040-05
作者:
郭超林12丁重阳12陆健12顾正华12张梁12石贵阳12
1. 江南大学 生物工程学院 工业生物技术教育部重点实验室,江苏 无锡 214122; 2. 江南大学 粮食发酵工艺与技术国家工程实验室,江苏 无锡 214122
Author(s):
GUO Chaolin12DING Zhongyang12LU Jian12GU Zhenghua12ZHANG Liang12SHI Guiyang12
1.Key Laboratory of Industrial Biotechnology of the Ministry of Education,School of Biotechnology,Jiangnan University, Wuxi 214122,China; 2.National Engineering Laboratory for Cereal Fermentation Technology,Jiangnan University,Wuxi 214122,China
关键词:
漆酶 共培养 促进物质 热处理酵母细胞 Pleurotus ferulae Rhodotorula mucilaginosa
分类号:
Q815
DOI:
10.3969/j.issn.1672-3678.2017.04.007
文献标志码:
A
摘要:
阿魏蘑在液态发酵过程中经胶红酵母共培养后,漆酶的产量得到提高,但对其起促进作用的物质尚不清楚。本文中,笔者首先考察阿魏蘑和胶红酵母发酵上清液混合对漆酶酶活测定的影响,发现胶红酵母并没有表达漆酶的能力,同时,发酵上清液的混合会导致漆酶酶活的下降。因此,排除了两种微生物发酵液之间发生的协同或促进作用对漆酶酶活的影响。在分别添加高温灭活和70 ℃灭活的胶红酵母后发现,添加30 g/L的70 ℃灭活的胶红酵母,阿魏蘑漆酶酶活增加至6 605 U/L,而高温灭活未有相应的促进效果。因此,在共培养中促进漆酶合成的活性物质存在于酵母中并对温度敏感。通过比较70 ℃灭活胶红酵母的添加时间和添加量后,在阿魏蘑培养第2天添加50 g/L胶红酵母获得最高漆酶产量7 579 U/L。通过研究发现了胶红酵母中某种对热敏感的物质促进了阿魏蘑漆酶表达量的提高,此结果对研究漆酶及其他发酵产物在共培养中表达量的提高有重要意义。

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备注/Memo

备注/Memo:
收稿日期:2016-03-31
基金项目:国家自然科学基金(31571822)
作者简介:郭超林(1990—),男,河南周口人,研究方向:发酵工学; 丁重阳(联系人),教授,E-mail:zyding@jiangnan.edu.cn; 陆健(联系人),教授,E-mail:jlu@jiangnan.edu.cn.
更新日期/Last Update: 2017-07-30