|本期目录/Table of Contents|

[1]宋灿辉,张伟国.敲除aceE基因对大肠杆菌生长和丙酮酸代谢的影响[J].生物加工过程,2013,11(06):15-18.[doi:10.3969/j.issn.1672-3678.2013.06.003]
　SONG Canhui,ZHANG Weiguo.Effects of aceE gene knockout on growing and pyruvate biosynthesis of E.coli[J].Chinese Journal of Bioprocess Engineering,2013,11(06):15-18.[doi:10.3969/j.issn.1672-3678.2013.06.003]
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敲除aceE基因对大肠杆菌生长和丙酮酸代谢的影响()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
11
期数:
2013年06期
页码:
15-18
栏目:
出版日期:
2013-11-30

文章信息/Info

Title:
Effects of aceE gene knockout on growing and pyruvate biosynthesis of E.coli
文章编号:
1672-3678(2013)06-0015-04
作者:
宋灿辉; 张伟国
江南大学 工业生物技术教育部重点实验室,无锡 214122
Author(s):
SONG Canhui; ZHANG Weiguo
Key Laboratory of Industrial Biotechnology of the Ministry of Education,Jiangnan University,Wuxi 214122, China
关键词:
aceE;  大肠杆菌;  丙酮酸;  Red重组
分类号:
Q814
DOI:
10.3969/j.issn.1672-3678.2013.06.003
文献标志码:
A
摘要:
大肠杆菌aceE基因是编码丙酮酸脱氢酶多酶复合体PdhR的关键酶之一。利用Red重组系统敲除大肠杆菌MG1655的aceE基因后,阻断了丙酮酸流向TCA循环,导致丙酮酸的累积,也使菌体生长受到影响,在培养基中补加5 g/L KAc后可以在一定程度上弥补菌株在生长上的缺陷。摇瓶发酵36 h,MG1655没有积累丙酮酸,MG1655ΔaceE∷cat菌株可以积累26.77 g/L丙酮酸,为利用大肠杆菌发酵生产丙酮酸奠定了基础。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2012-08-12
基金项目:国家高技术研究发展计划(863计划)(2008AA02Z212)
作者简介:宋灿辉(1986—),男,河南洛阳人,硕士研究生,研究方向:基因工程; 张伟国(联系人),教授,E-mail:zhangwg168@126.com.
更新日期/Last Update: 2013-11-30