|本期目录/Table of Contents|

[1]宋灿辉,张伟国.敲除aceE基因对大肠杆菌生长和丙酮酸代谢的影响[J].生物加工过程,2013,11(06):15-18.[doi:10.3969/j.issn.1672-3678.2013.06.003]
 SONG Canhui,ZHANG Weiguo.Effects of aceE gene knockout on growing and pyruvate biosynthesis of E.coli[J].Chinese Journal of Bioprocess Engineering,2013,11(06):15-18.[doi:10.3969/j.issn.1672-3678.2013.06.003]
点击复制

敲除aceE基因对大肠杆菌生长和丙酮酸代谢的影响()
分享到:

《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
11
期数:
2013年06期
页码:
15-18
栏目:
出版日期:
2013-11-30

文章信息/Info

Title:
Effects of aceE gene knockout on growing and pyruvate biosynthesis of E.coli
文章编号:
1672-3678(2013)06-0015-04
作者:
宋灿辉张伟国
江南大学 工业生物技术教育部重点实验室,无锡 214122
Author(s):
SONG CanhuiZHANG Weiguo
Key Laboratory of Industrial Biotechnology of the Ministry of Education,Jiangnan University,Wuxi 214122, China
关键词:
aceE 大肠杆菌 丙酮酸 Red重组
分类号:
Q814
DOI:
10.3969/j.issn.1672-3678.2013.06.003
文献标志码:
A
摘要:
大肠杆菌aceE基因是编码丙酮酸脱氢酶多酶复合体PdhR的关键酶之一。利用Red重组系统敲除大肠杆菌MG1655的aceE基因后,阻断了丙酮酸流向TCA循环,导致丙酮酸的累积,也使菌体生长受到影响,在培养基中补加5 g/L KAc后可以在一定程度上弥补菌株在生长上的缺陷。摇瓶发酵36 h,MG1655没有积累丙酮酸,MG1655ΔaceE∷cat菌株可以积累26.77 g/L丙酮酸,为利用大肠杆菌发酵生产丙酮酸奠定了基础。

参考文献/References:

[1] 张宗祥.采用微生物发酵制取丙酮酸的研究[D].南京:南京理工大学,2004.
[2] 刘立明,李寅,堵国成.生物技术法生产丙酮酸的研究进展[J].生物工程学报,2002,18(6):651-655.
[3] Ogasawara H,Ishida Y,Yamada K,et al.PdhR(pyruvate dehydrogenase complex regulator)controls the respiratory electron transport system in Escherichia coli[J].J Bacteriol,2007,189(5):5534-5541.
[4] 翁志明.用代谢工程构建高产番茄红素大肠杆菌[D].广州:中山大学,2010.
[5] Liu L M,Li Y,Du G C,et al.Breeding of high-pyruvate-producing Torulopsis glabrata with acetate as supplement catbon source[J].Ind Microbiol,2002,32(3):21-26.
[6] 杨松鑫,王蒙,汪军.基于光滑球拟酵母环境适应性的丙酮酸中试条件优化[J].过程工程学报,2011,11(6):1044-1049.
[7] 李迈.不同碳源及通气条件下lpdA基因敲除对大肠杆菌代谢的影响[J].化工学报,2006,57(4):880-885.
[8] 王佶.大肠杆菌积累丙酮酸的研究[D].杭州:浙江大学,2008.

相似文献/References:

[1]张琛,李环,吴圆丽,等.采用pHsh载体克隆与表达N-乙酰鸟氨酸脱乙酰基酶基因[J].生物加工过程,2012,10(05):67.[doi:10.3969/j.issn.1672-3678.2012.05.013]
 ZHANG Chen,LI Huan,WU Yuanli,et al.Cloning and high-level active expression of N-acetyl-L-ornithine deacetylase gene[J].Chinese Journal of Bioprocess Engineering,2012,10(06):67.[doi:10.3969/j.issn.1672-3678.2012.05.013]
[2]张旭,李宜奎,祁庆生.大肠杆菌碳分解代谢抑制及混合C源共利用的研究进展[J].生物加工过程,2014,12(01):109.[doi:10.3969/j.issn.1672-3678.2014.01.016]
 ZHANG Xu,LI Yikui,QI Qingsheng.Carbon catabolite repression and co-utilization of mixed carbon sources in Escherichia coli[J].Chinese Journal of Bioprocess Engineering,2014,12(06):109.[doi:10.3969/j.issn.1672-3678.2014.01.016]
[3]郑璐,柏中中,许婷婷,等.D-乳酸高产菌菊糖芽胞乳杆菌Y2-8磷酸果糖激酶基因在大肠杆菌中的克隆和表达[J].生物加工过程,2014,12(04):37.[doi:10.3969/j.issn.1672-3678.2014.04.008]
 ZHENG Lu,BAI Zhongzhong,XU Tingting,et al.Cloning and expression of phosphofructokinase gene from Sporolactobacillus inulinus in Escherichia coli[J].Chinese Journal of Bioprocess Engineering,2014,12(06):37.[doi:10.3969/j.issn.1672-3678.2014.04.008]
[4]袁春伟,何艳春,张胜利,等.重组大肠杆菌BL21(pUC19-Hyp)产羟脯氨酸的补料分批培养[J].生物加工过程,2014,12(04):43.[doi:10.3969/j.issn.1672-3678.2014.04.009]
 YUAN Chunwei,HE Yanchun,ZHANG Shengli,et al.Production of hydroxyproline by fed-batch culture of novel recombinant Escherichia coli BL21(pUC19-Hyp)[J].Chinese Journal of Bioprocess Engineering,2014,12(06):43.[doi:10.3969/j.issn.1672-3678.2014.04.009]
[5]梁丽亚,刘嵘明,苟冬梅,等.共表达烟酸转磷酸核糖激酶和丙酮酸羧化酶对大肠杆菌NZN111产丁二酸的影响[J].生物加工过程,2014,12(04):49.[doi:10.3969/j.issn.1672-3678.2014.04.010]
 LIANG Liya,LIU Rongming,GOU Dongmei,et al.Effects of co-expression of nicotinic acid phosphoribosyltransferase and pyruvate carboxylase on succinic acid production in Escherichia coli NZN111[J].Chinese Journal of Bioprocess Engineering,2014,12(06):49.[doi:10.3969/j.issn.1672-3678.2014.04.010]
[6]陈旭,梁丽亚,刘嵘明,等.共表达磷酸烯醇式丙酮酸羧激酶和烟酸转磷酸核糖激酶提高重组大肠杆菌发酵木糖产丁二酸[J].生物加工过程,2015,13(01):17.[doi:10.3969/j.issn.1672-3678.2015.01.004]
 CHEN Xu,LIANG Liya,LIU Rongming,et al.Enhancing succinate production from xylose by co-expression of phosphoenolpyruvate carboxykinase and nicotinic-acid phosphonbosyltransferase in recombinant Escherichia coli[J].Chinese Journal of Bioprocess Engineering,2015,13(06):17.[doi:10.3969/j.issn.1672-3678.2015.01.004]
[7]冯红茹,杨建明,秦利,等.β-蒎烯合成酶(QH6)在大肠杆菌中的表达及其产β-蒎烯的研究[J].生物加工过程,2015,13(01):28.[doi:10.3969/j.issn.1672-3678.2015.01.006]
 FENG Hongru,YANG Jianming,QIN Li,et al.Expression of β-pinene synthase(QH6)in Escherichia coli for the biosynthesis of β-pinene[J].Chinese Journal of Bioprocess Engineering,2015,13(06):28.[doi:10.3969/j.issn.1672-3678.2015.01.006]
[8]马红叶,郑仁朝,赵川东,等.重组大肠杆菌产疏绵状嗜热丝孢菌脂肪酶分批补料发酵工艺[J].生物加工过程,2015,13(02):9.[doi:10.3969/j.issn.1672-3678.2015.02.002]
 MA Hongye,ZHENG Renchao,ZHAO Chuandong,et al.Optimization of fed-batch fermentation for Thermomyces lanuginosus lipase production with recombinant Eschericha coli[J].Chinese Journal of Bioprocess Engineering,2015,13(06):9.[doi:10.3969/j.issn.1672-3678.2015.02.002]
[9]张汉文,刘嵘明,梁丽亚,等.大肠杆菌AFP111利用玉米粉全水解液厌氧发酵合成丁二酸[J].生物加工过程,2015,13(03):14.[doi:10.3969/j.issn.1672-3678.2015.03.003]
 ZHANG Hanwen,LIU Rongming,LIANG Liya,et al.Succinic acid production from complete hydrolysate of corn flour by anaerobic fermentation with Escherichia coli AFP111[J].Chinese Journal of Bioprocess Engineering,2015,13(06):14.[doi:10.3969/j.issn.1672-3678.2015.03.003]
[10]张凯,蔡恒,汪晨.通过DNA改组技术定向进化赖氨酸脱羧酶基因cadA和ldc[J].生物加工过程,2015,13(05):20.[doi:10.3969/j.issn.1672-3678.2015.05.004]
 ZHANG Kai,CAI Heng,WANG Chen.Directed evolution by DNA shuffling of lysine decarboxylase gene cadA and ldc[J].Chinese Journal of Bioprocess Engineering,2015,13(06):20.[doi:10.3969/j.issn.1672-3678.2015.05.004]

备注/Memo

备注/Memo:
收稿日期:2012-08-12
基金项目:国家高技术研究发展计划(863计划)(2008AA02Z212)
作者简介:宋灿辉(1986—),男,河南洛阳人,硕士研究生,研究方向:基因工程; 张伟国(联系人),教授,E-mail:zhangwg168@126.com.
更新日期/Last Update: 2013-11-30