|本期目录/Table of Contents|

[1]张琛,李环,吴圆丽,等.采用pHsh载体克隆与表达N-乙酰鸟氨酸脱乙酰基酶基因[J].生物加工过程,2012,10(05):67-71.[doi:10.3969/j.issn.1672-3678.2012.05.013]
 ZHANG Chen,LI Huan,WU Yuanli,et al.Cloning and high-level active expression of N-acetyl-L-ornithine deacetylase gene[J].Chinese Journal of Bioprocess Engineering,2012,10(05):67-71.[doi:10.3969/j.issn.1672-3678.2012.05.013]
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采用pHsh载体克隆与表达N-乙酰鸟氨酸脱乙酰基酶基因()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
10
期数:
2012年05期
页码:
67-71
栏目:
出版日期:
2012-09-30

文章信息/Info

Title:
Cloning and high-level active expression of N-acetyl-L-ornithine deacetylase gene
文章编号:
1672-3678(2012)05-0067-05
作者:
张琛李环吴圆丽韦萍
南京工业大学 生物与制药工程学院,南京 211800
Author(s):
ZHANG ChenLI HuanWU YuanliWEI Ping
College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 211800, China
关键词:
N-乙酰鸟氨酸脱乙酰基酶 大肠杆菌 克隆
分类号:
Q786
DOI:
10.3969/j.issn.1672-3678.2012.05.013
文献标志码:
A
摘要:
利用质粒pHsh为表达载体,构建高效表达N-乙酰鸟氨酸脱乙酰基酶的基因工程菌E.coli DH10B/argE-pHsh。为提高酶活并降低生产成本,优化了诱导条件。结果表明:NAOase可在pHsh系统中高活性表达,诱导起始OD600为0.6,在空气摇床中42 ℃热激诱导5 h重组菌比酶活达到152 U/mL。

参考文献/References:

[1] Javid-Majd F,Blanchard J S.Mechanistic analysis of the argE-encoded N-acetylornithine deacetylase[J].Biochem,2000,39(6):1285-1293.
[2] Vogel H J,Bonner D M.Acetylornithinase of Escherichia coli:partial purification and some properties[J].J Biolo Chem,1956,218:97-106.
[3] Thierry M,Emmanuelle S,Mechulam Y,et al.Structural and biochemical characterization of the Escherichia coli argE gene product[J].J Bacteriol,1992,174(7):2323-2331.
[4] Wade C,Mcgregor,Sabina I.ArgE-encoded N-acetyl-L-ornithine deacetylase from E.coli contains a dinuclear metalloactive site[J].J Am Chem Soc,2005,127(40):14100-14107.
[5] Boyen A,Charlier D,Charlier J,et al.Acetylornithine dacetylase succinyldiamino-pimelate desuccinylase and carboxypeptidase G2 are evolutionarily related[J].Gene,1992,116(1):1-6.
[6] 李环,陈悦,翁秋萍,等.重组N-乙酰鸟氨酸脱乙酰基酶的表达、纯化和复性研究[J].生物工程学报,2007,23(3):487-492.
[7] 吴伟华,邵蔚蓝.表达载体pHsh 对大肠杆菌热休克系统中负调控机制的影响[J].中国生物工程杂志,2009,29(6):79-84.

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备注/Memo

备注/Memo:
收稿日期:2011-12-30
基金项目:国家重点基础研究发展计划(973计划)资助项目(2003CB716004)
作者简介:张琛(1987—),女,江苏徐州人,硕士研究生,研究方向:发酵工程; 李环(联系人),副教授,E-mail:lihuan45678@163.com.
更新日期/Last Update: 2012-09-30