|本期目录/Table of Contents|

[1]付莉莉,关波,雷楗勇,等.人血清白蛋白在乳酸克鲁维酵母中的表达[J].生物加工过程,2012,10(05):55-60.[doi:10.3969/j.issn.1672-3678.2012.05.011]
 FU Lili,GUAN Bo,LEI Jianyong,et al.Expression of human serum albumin in Kluyveromyces lactis[J].Chinese Journal of Bioprocess Engineering,2012,10(05):55-60.[doi:10.3969/j.issn.1672-3678.2012.05.011]
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人血清白蛋白在乳酸克鲁维酵母中的表达()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
10
期数:
2012年05期
页码:
55-60
栏目:
出版日期:
2012-09-30

文章信息/Info

Title:
Expression of human serum albumin in Kluyveromyces lactis
文章编号:
1672-3678(2012)05-0055-06
作者:
付莉莉1关波1雷楗勇2段作营1陈蕴2金坚2李华钟1
1. 江南大学 生物工程学院 工业生物技术教育部重点实验室,无锡 214122; 2. 江南大学 医药学院,无锡 214122
Author(s):
FU Lili1GUAN Bo1LEI Jianyong2DUAN Zuoying1CHEN Yun2JIN Jian2LI Huazhong1
1. Key laboratory of Industrial Biotechnology of the Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China; 2. School of Medicine, Jiangnan University, Wuxi 214122, China
关键词:
人血清白蛋白 乳酸克鲁维酵母 分泌表达
分类号:
Q786
DOI:
10.3969/j.issn.1672-3678.2012.05.011
文献标志码:
A
摘要:
以乳酸克鲁维酵母(Kluyveromyces lactis,K.lactis)GG799为宿主对人血清白蛋白(HSA)进行分泌表达。以pPIC9k-HSA为模板,采用带有XhoⅠ和NotⅠ酶切位点的引物PCR扩增获得HSA基因,经XhoⅠ和NotⅠ双酶切后插入pKLAC1,构建表达载体pKLAC1-HSA。经SalⅡ线性化后,电击转化K.lactis GG799,用含5 mmol/L乙酰胺的YCB平板筛选阳性转化子。提取基因组DNA,采用PCR方法对转化子鉴定后进行摇瓶发酵。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)及Western blot分析发酵上清液中的表达产物,并初步分析酵母基础N源(YNB)对HSA在K.lactis GG799中表达的影响。结果表明,HSA成功在K.lactis GG799中分泌表达,表达量为81 μg/mL,遗传稳定性好。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2011-03-01
基金项目:国家自然科学基金资助项目(30970029)
作者简介:付莉莉(1986—),女,山东德州人,硕士研究生,研究方向:生物制药; 李华钟(联系人),教授,E-mail:hzhli@jiangnan.edu.cn.
更新日期/Last Update: 2012-09-30