|本期目录/Table of Contents|

[1]汤艳,郭亭,杜腾飞,等.响应面法优化甘蔗糖蜜发酵产丁醇[J].生物加工过程,2012,10(05):7-12.[doi:10.3969/j.issn.1672-3678.2012.05.002]
 TANG Yan,GUO Ting,DU Tengfei,et al.Optimization of butanol fermentation with cane molasses by response surface method[J].Chinese Journal of Bioprocess Engineering,2012,10(05):7-12.[doi:10.3969/j.issn.1672-3678.2012.05.002]
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响应面法优化甘蔗糖蜜发酵产丁醇()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
10
期数:
2012年05期
页码:
7-12
栏目:
出版日期:
2012-09-30

文章信息/Info

Title:
Optimization of butanol fermentation with cane molasses by response surface method
文章编号:
1672-3678(2012)05-0007-06
作者:
汤艳1郭亭12杜腾飞1张秋妍1陈啸鹏1姜岷1
1. 南京工业大学 生物与制药工程学院,南京 211800; 2. 广州甘蔗糖业研究所,广州 510316
Author(s):
TANG Yan1GUO Ting12DU Tengfei1ZHANG Qiuyan1CHEN Xiaopeng1JIANG Min1
1. College of Biotechnology and Pharmaceutical Engineering,Nanjing University of Technology, Nanjing 211800, China; 2. Guangzhou Sugarcane Industry Research Institute, Guangzhou 510316, China
关键词:
Clostridium beijerinckii 丁醇 糖蜜 优化
分类号:
TQ431.24
DOI:
10.3969/j.issn.1672-3678.2012.05.002
文献标志码:
A
摘要:
以甘蔗糖蜜为底物,用响应面法对高丁醇比突变菌株拜氏梭菌(Clostridium beijerinckii)ART124发酵生产丁醇的培养条件进行优化。首先利用Plackett-Burman试验设计筛选出影响丁醇生产的3个重要因素CaCO3和NH4HCO3和K2HPO4的用量,再通过最陡爬坡路径逼近最大响应区域,最后根据响应面中心组合设计理论,确定主要影响因素的最佳条件:CaCO3、NH4HCO3和K2HPO4的质量浓度分别为2.65、2.16 和0.43 g/L。利用数学模型分析预测得甘蔗糖蜜质量浓度为30 g/L时,最佳的丁醇产量为 8.10 g/L,比优化前提高了53.14%。在最佳工艺条件下得到的实验结果与模型预测值很吻合,说明所建立的模型是有效的。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2011-10-11
基金项目:国家重点基础研究发展计划(973计划)资助项目(2011CB707405); 材料化学工程国家重点实验室基金资助项目(ZK201001); 林业公益性专项基金资助项目(201004001); 国家自然科学基金青年基金资助项目(21106067); 江苏省高校自然科学研究重大项目(11KJA530001)
作者简介:汤艳(1987—),女,江苏盐城人,硕士研究生,研究方向:微生物学; 姜岷(联系人),教授,E-mail:biotechnology@njut.edu.cn.
更新日期/Last Update: 2012-09-30