|本期目录/Table of Contents|

[1]曾志鹏,张娟琨,刘珺,等.多孔硅Bragg反射镜适配子生物传感器检测心肌肌钙蛋白I[J].生物加工过程,2012,10(02):68-72.[doi:10.3969/j.issn.1672-3678.2012.02.014]
 ZENG Zhipeng,ZHANG Juankun,LIU Jun,et al. Detection of cardiac troponin I based on aptamer biosensor on nanoporous silicon Bragg reflector[J].Chinese Journal of Bioprocess Engineering,2012,10(02):68-72.[doi:10.3969/j.issn.1672-3678.2012.02.014]
点击复制

多孔硅Bragg反射镜适配子生物传感器检测心肌肌钙蛋白I()
分享到:

《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
10
期数:
2012年02期
页码:
68-72
栏目:
出版日期:
2012-03-30

文章信息/Info

Title:
 Detection of cardiac troponin I based on aptamer biosensor on nanoporous silicon Bragg reflector
文章编号:
1672-3678(2012)02-0068-05
作者:
曾志鹏1张娟琨1刘珺1王明伟1林青1徐慧静2
1.天津科技大学 生物工程学院 天津市工业微生物重点实验室 教育部工业微生物重点实验室,天津300457;
2. 天津市产品质量监督检测技术研究院,天津300384
Author(s):
ZENG Zhipeng1ZHANG Juankun1LIU Jun1WANG Mingwei1LIN Qing1 XU Huijing2
1. Key Laboratory of Industrial Microbiology of the Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Bioengineering, Tianjin University of Science and Technology, Tianjin 300457, China;

2.Tianjin Product Quality Inspection Technology Research Institute, Tianjin 300384, China

关键词:
适配子生物传感器多孔硅Bragg反射镜心肌肌钙蛋白I
分类号:
R392.11
DOI:
10.3969/j.issn.1672-3678.2012.02.014
文献标志码:
A
摘要:
通过脉冲腐蚀法制备多孔硅Bragg反射镜,将心肌肌钙蛋白I(cTnI)适配子共价固定到多孔硅Bragg反射镜的孔洞中,发现适配子能与cTnI分子特异性结合。定量分析不同浓度的cTnI与适配子结合后多孔硅Bragg反射镜的反射谱峰位的红移情况。结果表明:基于多孔硅Bragg反射镜适配子生物传感器的光学检测具有良好的特异性,且具有免标记及检测时间短等优异性能。传感器的线性检测范围0.05~4 nmol/L,最低检测限为0.05 nmol/L。

参考文献/References:

 [1]Eugene B,Raymond J,Elliott M,et al.ACC/AHA 2002 guidline update for the management of patients with unstable angina and nonSTsegment elevation myocardial infarction[J].J Am Colloid Cardiol,2002,40(7):1366-1374.

[2]王峰,向定成.肌钙蛋白I检测方法的研究进展[J].实用医学杂志,2008,24(8):1275-1277.
[3]Meskini O,Abdelghani A,Tlili A,et al.Porous silicon as functionalized material for immunosensor application[J].Talanta,2007,71(3):1430-1433.

[4]Lin V S Y,Motesharei K,Dancil K P S,et al.A porous siliconbased optical interferometric biosensor[J].Science,1997,278:840-843.

[5]DeLouise L A,Kou P M,Miller B L.Crosscorrelation of optical microcavity biosensor response with immobilized enzyme activity.Insights into biosensor sensitivity[J].Anal Chem,2005,77(10):3222-3230.

[6]Ouyang H M,Delouise L A,Miller B L,et al.Labelfree quantitative detection of protein using macroporous silicon photonicbandgap biosensors[J].Anal Chem,2007,79(4):1502-1506.

[7]赵晶,宋诗莹,张子怡,等.基于纳米多孔硅Bragg反射镜免标记生物传感器的研究[J].生物加工过程,2010,8(5):59-63.

[8]李志全,乔淑欣,蔡亚楠,等.基于多孔硅Bragg反射镜的液体浓度测量方法[J].光学技术,2006,32(6):863-864;868.

[9]吕小毅,庆格乐图,向梅,等.基于多孔硅Bragg反射镜的光学免疫检测方法[J].高等学校化学学报,2009,30(12):2381-2386.

[10]王忠东,李志全.基于多孔硅反射镜的农药浓度测量实验研究[J].化工自动化及仪表,2011(4):392-395.

[11]王忠东,常胜江,王志彬,等.基于多孔硅反射谱的农药浓度测量研究[J].光学技术,2011,37(3):332-335.

[12]Qi Yongzhi,Yao Chunyan,Luo Yang,et al.Aptamerbased piezoelectric quartz crystal biosensor[J].Chin J Nosocomiol,2008,18 (3):353-355.

[13]Hunkel D,Butz R,AresFisher R,et al.Interference flters from porous silicon with laterally varying wavelength of reflection[J].J Lumin,1998,80:133-136.

[14]张彬彬,张娟琨,曾志鹏,等.多孔硅适配子生物传感器的电化学研究[J].生物加工过程,2011,9(1):61-65.

[15]董杉木,张娟琨,白丽荣,等.纳米多孔硅阻抗生物传感器的研究[J].生物加工过程,2009,7(4):56-60.

[16]刘盛辉,孙长林,何品刚,等.单链脱氧核糖核酸在石墨电极表面固定化的研究[J].分析化学,1999,27(2):130-134.

[17]Hermanson G T.Bioconjugate techniques[M].2ed.Rockford:Academic Press,2008.

[18]Ouyang H M,Striemer C C,Fauchet P M.Quantitative analysis of the sensitivity of porous silicon optical biosensors[J].Appl Phys Lett,2006,88(16):163108.



相似文献/References:

[1]张彬彬,张娟琨,曾志鹏,等.多孔硅适配子生物传感器的电化学研究[J].生物加工过程,2011,9(01):55.[doi:10.3969/j.issn.1672-3678.2011.01.013]
 ZHANG Binbin,ZHANG Juankun,ZENG Zhipeng,et al.Electrochemical study of aptamer biosensor on nano porous silicon[J].Chinese Journal of Bioprocess Engineering,2011,9(02):55.[doi:10.3969/j.issn.1672-3678.2011.01.013]
[2]李成学,张娟琨,蒋树海,等.心肌肌钙蛋白I的分子印迹电化学生物传感器[J].生物加工过程,2012,10(03):61.[doi:10.3969/j.issn.1672-3678.2012.03.012]
 LI Chengxue,ZHANG Juankun,JIANG Shuhai,et al.Electrochemical biosensor of molecular imprinting with cardiac troponin I[J].Chinese Journal of Bioprocess Engineering,2012,10(02):61.[doi:10.3969/j.issn.1672-3678.2012.03.012]
[3]潘玉宁,颜春荣,张蕾,等.基于纳米材料的生物传感技术在食品安全检测中的应用[J].生物加工过程,2018,16(02):17.[doi:10.3969/j.issn.1672-3678.2018.02.003]
 PAN Yuning,YAN Chunrong,ZHANG Lei,et al.Application of nono-based biosensors in food safty assessment[J].Chinese Journal of Bioprocess Engineering,2018,16(02):17.[doi:10.3969/j.issn.1672-3678.2018.02.003]
[4]范婷,张娟琨,李敏,等.适配子生物传感器对青霉素的快速检测[J].生物加工过程,2011,9(03):41.[doi:10.3969/j.issn.1672-3678.2011.03.009]
 FAN Ting,ZHANG Juankun,LI Min,et al.Quick detection of penicillin by aptamer biosensor[J].Chinese Journal of Bioprocess Engineering,2011,9(02):41.[doi:10.3969/j.issn.1672-3678.2011.03.009]
[5]孙坤,张娟琨,徐培东,等.适配子纳米生物传感器在青霉素检测中的应用[J].生物加工过程,2011,9(04):58.[doi:10.3969/j.issn.1672-3678.2011.04.013]
 SUN Kun,ZHANG Juankun,XU Peidong,et al.Application of aptamer nanobiosensor in penicillin detection[J].Chinese Journal of Bioprocess Engineering,2011,9(02):58.[doi:10.3969/j.issn.1672-3678.2011.04.013]

备注/Memo

备注/Memo:
收稿日期:2011-09-09

基金项目:科技部资助项目(2009GJA10047);天津市科委资助项目(07ZHZHNC04500,09ZCZDSF04200)

作者简介:曾志鹏(1986—),男,江西吉安人,硕士研究生,研究方向:纳米生物传感器;张娟琨(联系人),教授,E-mail:zhangjk@tust.edu.cn



更新日期/Last Update: