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[1]魏淼,刘欢,李艳,等.共表达甘油脱氢酶和二羟丙酮激酶对大肠杆菌生长及甘油代谢的影响[J].生物加工过程,2011,9(05):59-64.[doi:doi:10.3969/j.issn.1672-3678.2011.05.012]
 WEI Miao,LIU Huan,LI Yan,et al.Effects on growth and glycerol metabolism in E.coli by coexpression protein GldA and DhaKLM[J].Chinese Journal of Bioprocess Engineering,2011,9(05):59-64.[doi:doi:10.3969/j.issn.1672-3678.2011.05.012]
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共表达甘油脱氢酶和二羟丙酮激酶对大肠杆菌生长及甘油代谢的影响()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
9
期数:
2011年05期
页码:
59-64
栏目:
出版日期:
2011-09-30

文章信息/Info

Title:
Effects on growth and glycerol metabolism in E.coli by coexpression protein GldA and DhaKLM
文章编号:
1672-3678(2011)05-0059-06
作者:
魏淼刘欢李艳严明许琳
南京工业大学 生物与制药工程学院,南京210009
Author(s):
WEI MiaoLIU HuanLI YanYAN MingXU Lin
College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China
关键词:
二羟丙酮激酶大肠杆菌甘油脱氢酶
分类号:
Q78
DOI:
doi:10.3969/j.issn.1672-3678.2011.05.012
文献标志码:
A
摘要:
考察共表达甘油脱氢酶(GldA)和二羟丙酮激酶(DhaKLM)对大肠杆菌生长及甘油代谢的影响。结果表明:在好氧条件下,共表达甘油脱氢酶及二羟丙酮激酶可以提高大肠杆菌利用甘油合成菌体的效率,利用等量的甘油,重组菌最高菌密度比对照菌提高了70%,细胞干质量为3.54 g(以每升发酵液计)。在厌氧条件下,仅共表达甘油脱氢酶并不能促进大肠杆菌的甘油代谢,而同时共表达甘油脱氢酶和二羟丙酮激酶可以明显提高大肠杆菌代谢甘油的能力,每克菌体消耗的甘油量提高了42%,每克干细胞中达11.08 g,代谢产物组成也发生显著变化,乙酸成为主要产物。这说明共表达gldAdhaKLM基因能有效促进大肠杆菌好氧利用甘油生长及厌氧甘油代谢的能力。

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备注/Memo

备注/Memo:
收稿日期:2011-03-25
基金项目:国家重点基础研究发展计划(973计划)资助项目(2009CB724700)
作者简介:魏淼(1984—) ,男,江苏徐州人,硕士研究生,研究方向:生物化工;李艳(联系人),讲师,E-mail:liyan@njut.edu.cn
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