|本期目录/Table of Contents|

[1]郝继伟,邱奉同.响应面法优化松茸发酵产多糖的培养条件[J].生物加工过程,2011,9(04):1-5.[doi:10.3969/j.issn.1672-3678.2011.04.001]
 HAO Jiwei,QIU Fengtong.Optimization of fermentation conditions for polysaccharide of Tricholoma matsutake using response surface methodology[J].Chinese Journal of Bioprocess Engineering,2011,9(04):1-5.[doi:10.3969/j.issn.1672-3678.2011.04.001]
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响应面法优化松茸发酵产多糖的培养条件()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
9
期数:
2011年04期
页码:
1-5
栏目:
出版日期:
2011-07-30

文章信息/Info

Title:
Optimization of fermentation conditions for polysaccharide of Tricholoma matsutake using response surface methodology
文章编号:
1672-3678(2011)04-0001-05
作者:
郝继伟邱奉同
临沂大学生命科学学院,临沂276005
Author(s):
HAO JiweiQIU Fengtong
College of Life Science, Linyi University, Linyi 276005, China
关键词:
实验设计发酵多糖松茸
分类号:
TQ353
DOI:
10.3969/j.issn.1672-3678.2011.04.001
文献标志码:
A
摘要:
运用响应面法对松茸产多糖的发酵培养条件进行优化研究。首先根据C、N源实验结果,利用PlackettBurman设计,对影响多糖产量的相关因素进行评估,筛选出具有显著效应的3个因素:玉米粉、豆粕和KH2PO4。在此基础上,利用最陡爬坡试验逼近以上3个因素的最大响应区域,采用BoxBehnken设计法对各因素的水平组合进行优化,获得松茸产多糖优化发酵的培养条件:玉米粉质量分数454%,豆粕质量分数496%,KH2PO4质量分数015%,MgSO4·7H2O质量分数005%,VB1质量分数0001%,初始pH 55,摇床转速180 r/min,发酵时间10 d。在此优化培养条件下松茸总多糖产量可达597 g/L。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2011-04-03
基金项目:临沂大学社会立项课题项目(2010ly0401)
作者简介:郝继伟(1971—),男,山东临沂人,副教授,研究方向:食品营养与天然产物有效成分的提取,Email:haojw@126.com
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