|本期目录/Table of Contents|

[1]吕浩,崔丁维,胡学超,等.酶法破碎裂殖壶菌提取胞内油脂[J].生物加工过程,2011,9(03):27-30.[doi:10.3969/j.issn.1672-3678.2011.03.006]
 Lv Hao,CUI Dingwei,HU Xuechao,et al.Extraction of oil from Schizochytrium sp by enzymatic hydrolysis[J].Chinese Journal of Bioprocess Engineering,2011,9(03):27-30.[doi:10.3969/j.issn.1672-3678.2011.03.006]
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
9
期数:
2011年03期
页码:
27-30
栏目:
出版日期:
2011-05-30

文章信息/Info

Title:
Extraction of oil from Schizochytrium sp by enzymatic hydrolysis
文章编号:
1672-3678(2011)03-0027-04
作者:
吕浩1崔丁维1胡学超2张红漫3
1. 南京工业大学 生物与制药工程学院,南京210009; 2. 江苏省工业生物技术创新中心, 南京211816; 3. 南京工业大学 理学院, 南京210009
Author(s):
?Lv Hao1CUI Dingwei1HU Xuechao2ZHANG Hongman3
?1. College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China;
2. Jiangsu Provincal Innovation Center for Industrial Biotechnology, Nanjing 211816, China;
3. College of Sciences, Nanjing University of Technology, Nanjing 210009, China
关键词:
裂殖壶菌 酶法 水解提油量
分类号:
TQ641
DOI:
10.3969/j.issn.1672-3678.2011.03.006
文献标志码:
A
摘要:
采用酶法破碎裂殖壶菌提取胞内油脂, 进行单因素实验和正交实验优化酶解反应条件,酶解反应的影响因素主次顺序依次为酶用量、温度、时间、pH, 最佳酶解工艺参数:55 ℃、pH 9.5、搅拌反应2.5 h、酶用量为菌体生物量的2%。在该条件下,胞内油脂的提取量高达(81.53±0.33) g/L,过氧化值仅为0.15,酸价为0.24。

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备注/Memo

备注/Memo:
?收稿日期:2010-12-02
基金项目:国家自然科学基金重点资助项目(20936002);国家自然科学基金资助项目(20876078);国家科技支撑计划资助项目(2011BAD23B03)
作者简介:吕浩(1970—),男,江苏南京人,硕士,高级工程师,研究方向:生物分离工程、酶工程,E-mail:sanvango@njut.edu.cn
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