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[1]李辉,王义强,陈介南,等.工业纤维素诱导里氏木霉RUT C-30产葡聚糖酶的条件[J].生物加工过程,2011,9(03):22-26.[doi:10.3969/j.issn.1672-3678.2011.03.005]
 LI Hui,WANG Yiqiang,CHEN Jienan,et al.Conditions of glucanase production for Trichoderma reesei RUT C-30[J].Chinese Journal of Bioprocess Engineering,2011,9(03):22-26.[doi:10.3969/j.issn.1672-3678.2011.03.005]
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
9
期数:
2011年03期
页码:
22-26
栏目:
出版日期:
2011-05-30

文章信息/Info

Title:
Conditions of glucanase production for Trichoderma reesei RUT C-30
文章编号:
1672-3678(2011)03-0022-05
作者:
李辉王义强陈介南张伟涛
?中南林业科技大学生物环境科学与技术研究所,长沙410004
Author(s):
?LI HuiWANG YiqiangCHEN JienanZHANG Weitao
?Institute of Biological and Environmental Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China
关键词:
里氏木霉纤维素葡聚糖酶响应面方法中心组合设计
分类号:
Q815
DOI:
10.3969/j.issn.1672-3678.2011.03.005
文献标志码:
A
摘要:
?为了研究工业纤维素诱导里氏木霉RUT C-30产葡聚糖酶的最佳条件,根据单因素实验结果,以工业纤维素、(NH42SO4和生物素为实验因素,滤纸酶活为响应值,进行中心组合设计,建立一个二次多项式数学模型,进行响应面优化,寻找最优产酶结果。经过优化,选出工业纤维素、(NH42SO4和生物素的添加量分别为39.485 g/L、6.232 g/L和249.872 μg/L,最高的滤纸比酶活为6.298 U/mL,实验验证,滤纸比酶活为6.118 U/mL,与预测值相差了2.86%。

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备注/Memo

备注/Memo:
?收稿日期:2010-12-28
基金项目:国家林业局“948”引进项目(2006-4-123);国家林业公益性行业科研专项资助项目(201004001);常德市科学技术局技术研究与开发资金项目(2010BS04)
作者简介:李辉(1986—),男,河南商丘人,硕士研究生,研究方向:发酵工程、纤维素酶生产;王义强(联系人),教授,博士生导师,E-mail:wangyiqiang12@yahoo.com.cn
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