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[1]李敏,张娟琨,张子怡,等.分子印迹技术应用于血清中地高辛的快速检测[J].生物加工过程,2011,9(02):64-67.[doi:doi:10.3969/j.issn.1672-3678.2011.02.013]
 LI Min,ZHANG Juankun,ZHANG Ziyi,et al.Rapid detection of digoxin in serum samples by molecular imprinted biosensor[J].Chinese Journal of Bioprocess Engineering,2011,9(02):64-67.[doi:doi:10.3969/j.issn.1672-3678.2011.02.013]
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分子印迹技术应用于血清中地高辛的快速检测
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
9
期数:
2011年02期
页码:
64-67
栏目:
出版日期:
2011-03-30

文章信息/Info

Title:
Rapid detection of digoxin in serum samples by molecular imprinted biosensor
文章编号:
1672-3678(2011)02-0064-04
作者:
李敏1张娟琨1张子怡2张彬彬1范婷1吴萍1
1. 天津科技大学? 生物工程学院? 工业微生物教育部重点实验室,天津? 300457;
2. 天津体育学院? 运动人体科学系,天津? 300381
Author(s):
LI Min1ZHANG Juankun1ZHANG Ziyi2ZHANG Binbin1FAN Ting1WU Ping1
1. Key Laboratory of Industrial Microbiology of the Ministry of Education,College of Bioengineering,??Tianjin University of Science and Technology,Tianjin? 300457,China;
2. Department of Health and Exercise Science,Tianjin University of Sport,Tianjin 300381,China
关键词:
分子印迹传感器地高辛聚合物血清快速检测
分类号:
TQ212.3
DOI:
doi:10.3969/j.issn.1672-3678.2011.02.013
文献标志码:
A
摘要:
应用分子印迹的方法制备对地高辛有特异性吸附性能的印迹聚合物颗粒,再将颗粒与琼脂糖混合并固定于玻碳电极上制备成地高辛分子印迹聚合膜传感器,传感器可以特异性地结合模板分子地高辛且其电化学信号与模板浓度相关,再用它来检测血清中地高辛的含量。结果表明:分子印迹传感器具有制作简便、成本低、检测快速、特异性高、稳定性好等优点,检测下限为1.28 nmol/L,检测时间为5 min。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2010-06-12
基金项目:科技部资助项目(2009GJA10047);天津市科委资助项目(08ZXCXSH053,09ZCZDSF04200)
作者简介:李敏(1985—),女,内蒙古包头人,硕士研究生,研究方向:电化学生物传感器;张娟琨(联系人),教授,博士生导师,E-mail:zhangjk@tust.edu.cn
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