|本期目录/Table of Contents|

[1]吴勋贵,王泽根,蔡寅,等. 苦瓜多糖的提取、分离纯化及组成性质[J].生物加工过程,2011,9(01):19-23.[doi:10.3969/j.issn.1672-3678.2011.01.005]
 WU Xungui,WANG Zegen,CAI Yin,et al.Extraction, isolation, purification and composition of Momordica charantia polysaccharide[J].Chinese Journal of Bioprocess Engineering,2011,9(01):19-23.[doi:10.3969/j.issn.1672-3678.2011.01.005]
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 苦瓜多糖的提取、分离纯化及组成性质()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
9
期数:
2011年01期
页码:
19-23
栏目:
出版日期:
2011-01-20

文章信息/Info

Title:
Extraction, isolation, purification and composition of Momordica charantia  polysaccharide
文章编号:
1672-3678(2011)01-0019-05
作者:
吴勋贵王泽根蔡寅杨烨王旻
中国药科大学生命科学与技术学院, 南京210009
Author(s):
WU XunguiWANG ZegenCAI YinYANG YeWANG Min
College of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China
关键词:
苦瓜多糖分离纯化单糖衍生化
分类号:
Q539
DOI:
10.3969/j.issn.1672-3678.2011.01.005
文献标志码:
A
摘要:
正交实验确定提取工艺后,用热水提取法得到苦瓜多糖(MCP)。对MCP进行DEAE32离子交换层析分离,得到3个多糖组分MCP1、MCP2和MCP3。 进一步采用Sephacryl S400凝胶层析进行分离,经凝胶层析和高效液相色谱检测表明,MCP1、MCP2为均一性多糖组分。通过高效液相凝胶色谱法测定了两者的相对分子质量分别为116×106和745×105。用PMP衍生化法测定其单糖,结果表明: MCP1系由Man、Rham、GlcUA、GalUA、Glu、Gal、Xyl、Ara等单糖组成的杂多糖,摩尔比为103∶293∶100∶1495∶216∶3070∶285∶450。MCP2系由Rham、GalUA、Gal、Xyl、Ara等单糖组成的杂多糖,对应的摩尔比为163∶2188∶466∶100∶129。紫外光谱表明该多糖不含蛋白质和核酸。

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备注/Memo

备注/Memo:
收稿日期:2010-07-29
基金项目:江苏省六大人才高峰资助项目
作者简介:吴勋贵(1986—),男,贵州遵义人,硕士研究生,研究方向:微生物与生化药学;王旻(联系人),教授,博士生导师,Email:minwang@cpu.edu.cn
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