|本期目录/Table of Contents|

[1]孙坤,张娟琨,徐培东,等.适配子纳米生物传感器在青霉素检测中的应用[J].生物加工过程,2011,9(04):58-63.[doi:10.3969/j.issn.1672-3678.2011.04.013]
 SUN Kun,ZHANG Juankun,XU Peidong,et al.Application of aptamer nanobiosensor in penicillin detection[J].Chinese Journal of Bioprocess Engineering,2011,9(04):58-63.[doi:10.3969/j.issn.1672-3678.2011.04.013]
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适配子纳米生物传感器在青霉素检测中的应用()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
9
期数:
2011年04期
页码:
58-63
栏目:
出版日期:
2011-07-30

文章信息/Info

Title:
Application of aptamer nanobiosensor in penicillin detection
文章编号:
1672-3678(2011)04-0058-06
作者:
孙坤张娟琨徐培东王明伟林青徐锦锋宋可
天津科技大学生物工程学院天津市工业微生物重点实验室教育部工业微生物重点实验室,天津300457
Author(s):
SUN Kun ZHANG Juankun XU Peidong WANG Mingwei LIN Qing XU Jinfeng SONG Ke
?Key Laboratory of Industrial Microbiology of the Ministry of Education,Tianjin Key Laboratory of Industrial Microbiology,
?College of Bioengineering, Tianjin University of Science and Technology, Tianjin 300457, China
关键词:
适配子生物传感器多孔硅
分类号:
R39211
DOI:
10.3969/j.issn.1672-3678.2011.04.013
文献标志码:
A
摘要:
通过脉冲腐蚀法对硅片进行多孔硅的制备,利用玻片通过对共价法、离子吸附法和APTES修饰的戊二醛交联法3种固定适配子方法的对比,以确定较好的固定青霉素适配子的方法。将适配子固定在多孔硅上后,利用交流阻抗法对加入青霉素前后传感器阻抗值进行测定、对比,构建等效电路并进行阻抗拟合。对多孔硅传感器的Nyquist谱图进行分析以确定多孔硅表面成功固定了青霉素适配子,从而证明构建纳米生物传感器成功。传感器的线性检测范围为005~02 mg/L,检测限为005 mg/L。

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备注/Memo

备注/Memo:
收稿日期:2011-05-03
基金项目:科技部资助项目(2009GJA10047);天津市科委资助项目(07ZHXHNC04500,09ZCZDSF04200)
作者简介:孙坤(1985—),男,山东淄博人,硕士研究生,研究方向:纳米生物传感器;张娟琨(联系人),教授,博士生导师,Email:zhangjk@tust.edu.cn
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