|本期目录/Table of Contents|

[1]范林萍,杨顺楷,吴中柳,等.双菌多轮序列协同转化甾体制氢化可的松[J].生物加工过程,2009,7(06):15-20.
 FAN Lin-ping,YANG Shun-kai,WU Zhong-liu,et al.A novel process of multi-run sequence bioconversion of RSA for production of hydrocortisone with Absidia coerulea AS 3.65 and Curvularia lunata AS 3.4381[J].Chinese Journal of Bioprocess Engineering,2009,7(06):15-20.
点击复制

双菌多轮序列协同转化甾体制氢化可的松
(/HTML)
分享到:

《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
7
期数:
2009年06期
页码:
15-20
栏目:
出版日期:
2009-11-30

文章信息/Info

Title:
A novel process of multi-run sequence bioconversion of RSA for production of hydrocortisone with Absidia coerulea AS 3.65 and Curvularia lunata AS 3.4381
文章编号:
1672-3678(2009)06-0015-06
作者:
范林萍1杨顺楷2吴中柳2杨亚力2李小刚1
1. 兰州大学 生命科学院,兰州730000;
2. 中国科学院 成都生物研究所,成都610041
Author(s):
FAN Lin-ping1 YANG Shun-kai2 WU Zhong-liu2 YANG Ya-li2 LI Xiao-gang1
1. School of Life Sciences, Lanzhou University, Lanzhou 730000, China;
2. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
关键词:
蓝色犁头霉新月弯孢霉多轮序列生物转化氢化可的松
分类号:
TQ467.8
文献标志码:
A
摘要:
建立了蓝色犁头霉AS 3.65和新月弯孢霉AS 3.4381协同多轮转化17α-羟基-孕甾-4-烯-3,20-二酮-21-醋酸酯(RSA)制氢化可的松(hydrocortisone,HC)新工艺。在培养好的AS 3.65和AS 3.4381所组成的协同转化体系中,AS 3.65首先将RSA水解为脱氧皮质酮(RS),这较AS 3.4381单轮批次转化省去了RSA到RS的化学水解工序。在甾体底物RSA平均投料质量浓度为1.3 g/L和1 g/L的条件下,所选定的协同转化体系可分别被重复利用3轮和6轮,相应的平均产率能维持在较高水平,分别高达81.6%和85%。另外,该工艺明显减少了底物RSA投料浓度对C11位羟化的影响,并有效抑制了AS 3.4381和AS 3.65单独转化过程中出现的14α-OH-RS和11α-OH-RS副产物。

参考文献/References:

[1]张丽青,禇志义.甾体微生物转化,甾体化学进展[M].北京:科学出版社,2002:346-360.
[2]Spalla C,Modelli R,Amici A M.Production of hydrocortisone by multiple fermentation: US,3030278[P].1962-04-17.
[3]Mazumder T K,Sonomoto K,Tanaka A,et al.Sequential conversion of cortexolone to prednisolone by immobilized mycelia of Curvularia lunata and immobilized
cells of Arthrobacter simplex[J].Applied Microbiology Biotechnology,1985,21:154-161.
[4]法幼华,马树恒,苏起恒,等.转化5α-娠烷-3β,17α-二羟基-20-酮-21-醋酸酯为氢化可的松(诺卡式菌62-1菌株和蓝色梨头霉AS 3.65的协同转化)[J].微生物学报,1981,21(4):489-492.
Fa Youhua,Ma Shuheng,Su Qiheng,et al.Conversion of 5α-pregenane-3β,17α-diol-20one-21-acetate to hydrocortisone an associated transformation of
Nocardia sp.62-1 and Absidia coerulea AS 3.65[J].Acta Microbiologica Sinica,1981,21(4):489-492.
[5]陈家任,蒲自莲,曾本秀,等.微生物学方法制备16α-甲基11α,17α,21-三羟基-孕甾-1,4-二烯-3,20-二酮[J].微生物学报,1991,31(4):308-314.
Chen Jiaren,Pu Zilian,Zeng Benxiu,et al.Microbiological preparation of 16α-methyl-11α,17β,21-trihydroxyl-pregn-1,4-diene-3,20-dione[J].Acta Microbiologica Sinica,1991,31(4):308-314.
[6]林新国,周才赋.犁头霉菌生产11β羟基化化合物RS-21醋酸酯的研究[J].化学反应工程与工艺,1995,11(2):213-216.
Lin Xinguo,Zhou Caifu.The synthesis of 11β-hydroxy compound RS-21 acetate by Absidia orchidis[J].Chemical Reaction Engineering and Technology,1995,11(2):213-216.
[7]Wilmanska D,Milcazarek K,Rumijiowska A,et al.Elimination of by-products in 11β-hydroxylation of substance S using Curvularia lunata clones ration from NTG-treatd protoplasts[J].Applied Microbiology Biotechnology,1992,37(5):626-630.
[8]Paraszkiewicz K,Dlugonski J.Cortexolone 11β-hydroxylation in protoplasts of Curvularia lunata[J].Journal of Biotechnology,1998,65(2/3):217-224.
[9]易奎星,杨亚力,杨顺楷,等.静息细胞连续两批次生物催化生产氢化可的松[J].生物加工过程,2005,3(4):40-44.
Yi Kuixing,Yang Yali,Yang Shunkai,et al.Bioconversion of compound S into hydrocortisone using a novel process of twi-run sequence bioconversion by the
mycelium of Curvularia lunata AS 3.4381[J].Chinese Journal of Bioprocess Engineering,2005,3(4):40-44.
[10]易奎星,杨亚力,杨顺楷,等.β-环糊精包合技术在微生物转化生产HC中的应用[J].中国医药工业杂志,2006,37(5):311-314.
Yi Kuixing,Yang Yali,Yang Shunkai,et al.Application of β-cyclodextrin inclusion technique in the biotransformation of hydrocortisone[J].Chinese Journal of harmaceuticals,2006,37(5):311-314.
[11]杨顺楷,易奎星,杨亚力,等.甾体微生物转化C11β-羟基化的研究进展[J].生物加工过程,2006,4(2):7-14.
Yang Shunkai,Yi Kuixing,Yang Yali,et al.Precess of study on C11β-hydroxylation of steroid by microbial transformation[J].Chinese Journal of Bioprocess ngineering,2006,4(2):7-14.
[12]郭亚文.新月弯孢霉抗性菌株筛选选育及P450酶特性分析[D].天津:天津科技大学,2004.
[13]Mogill′nitskii G M,Koshcheenko K A,Ponomareva E N,et al.Microbiological transformation of Reichstein′s substances and its 21-acetate under conditions of periodic addition to the initial steroid[J].Khimiko-Farmatsevticheskii Zhurnal,1977,11:94-99.
[14]Fu X Y,Tann C H,Thiruvengadam T K,et al.A regioselective PCl5 mediated dehydration for preparing Δ 9,11 corticosteroids[J].Tetrahedron Letters,2001,42:2639-2642.

相似文献/References:

[1]易奎星,杨亚力,杨顺楷,等.静息细胞连续两批次生物催化生产氢化可的松[J].生物加工过程,2005,3(04):40.[doi:10.3969/j.issn.1672-3678.2005.04.009]
 YI Kui-xing,YANG Ya-li,YANG Shun -kai,et al.Bioconversion of compound S into hydrocortisone using a novel process of twi-run sequence bioconversion by the mycelium of Curvularia lunata AS 3.4381[J].Chinese Journal of Bioprocess Engineering,2005,3(06):40.[doi:10.3969/j.issn.1672-3678.2005.04.009]
[2]张天智,杨炯,杨顺楷,等.新月弯孢霉AS 3.4381对新型甾体底物C11β-羟基化[J].生物加工过程,2006,4(03):22.[doi:10.3969/j.issn.1672-3678.2006.03.004]
 ZHANG Tian-zhi,YANG Jong,YANG Shun-kai,et al.Study on the biotransformation of a new steroid substrate into C11β-hydroxylated product by Curvularia lunata AS3.4381[J].Chinese Journal of Bioprocess Engineering,2006,4(06):22.[doi:10.3969/j.issn.1672-3678.2006.03.004]
[3]范林萍,杨顺楷,吴中柳,等.改进底物添加体系制备氢化可的松[J].生物加工过程,2010,8(06):15.[doi:10.3969/j.issn.1672-3678.2010.06.004]
 FAN Lin-ping,YANG Shun-kai,WU Zhong-liu,et al.Biotransformation RSA to hydrocortisone:improvement of adding substrate[J].Chinese Journal of Bioprocess Engineering,2010,8(06):15.[doi:10.3969/j.issn.1672-3678.2010.06.004]
[4]杨顺楷,易奎星,杨亚力,等.甾体微生物转化C11β-羟基化的研究进展[J].生物加工过程,2006,4(02):7.[doi:10.3969/j.issn.1672-3678.2006.02.002]
 YANG Shun-kai,YI Kui-xing,YANG Ya-li,et al.Progress of study on C11β-hydroxylation of steroid by microbial transformation[J].Chinese Journal of Bioprocess Engineering,2006,4(06):7.[doi:10.3969/j.issn.1672-3678.2006.02.002]

备注/Memo

备注/Memo:
收稿日期:2009-03-03
基金项目:中科院百人计划资助项目;四川省青年科技基金资助项目(08ZQ026023);云南丽江映华生物药业有限公司资助项目
作者简介:范林萍(1985—),女,四川中江人,硕士研究生,研究方向:应用微生物;杨顺楷(联系人),研究员, E-mail:yangsk@cib.ac.cn;吴中柳(联系人),研究员,E-mail:wuzhl@cib.ac.cn

更新日期/Last Update: