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[1]孙爱友,秦鲁敏,宋建民,等.乳酸片球菌素(Pediocin)效价测定的条件优化[J].生物加工过程,2009,7(05):69-72.
 SUN Ai-you,QIN Lu-min,SONG Jian-min,et al.Optimization of key factors in the measurement of Pediocin of potency[J].Chinese Journal of Bioprocess Engineering,2009,7(05):69-72.
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乳酸片球菌素(Pediocin)效价测定的条件优化
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
7
期数:
2009年05期
页码:
69-72
栏目:
出版日期:
2009-09-30

文章信息/Info

Title:
Optimization of key factors in the measurement of Pediocin of potency
文章编号:
1672-3678(2009)05-0069-04
作者:
孙爱友秦鲁敏宋建民董玉国魏东芝
华东理工大学 生物工程学院 生物反应器工程国家重点实验室,上海200237
Author(s):
SUN Ai-you QIN Lu-min SONG Jian-min DONG Yu-guo WEI Dong-zhi
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
关键词:
乳酸片球菌素效价测定
分类号:
Q331
文献标志码:
A
摘要:
目前的乳酸片球菌素(Pediocin)效价测定方法误差大、耗时较长。为了减少测定误差并缩短测定时间,研究并优化了乳酸片球菌素(Pediocin)效价测定中的几个关键因素,得到较优的实验条件:发酵液加热时间30 min、菌体吸附2 h、解吸附3 h、指示菌加入量为400 μL(30 mL固体检测培养基中含菌2.1×108个)、试样pH为2.0时,测定的Pediocin效价误差较小;试样处理时间由12 h缩短为约5 h。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2009-01-21
基金项目:国家自然科学基金资助项目(20706017)
作者简介:孙爱友(1975—),男,山东诸城人,讲师,博士,研究方向:生物化工,E-mail:sunaiyou@ecust.edu.cn
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