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[1]李丽敏,邹树平,戴大章,等.响应面设计法优化单葡萄糖醛酸基甘草次酸(GAMG)发酵转化培养基[J].生物加工过程,2009,7(05):29-33.
 LI Li-min,ZOU Shu-ping,DAI Da-zhang,et al.Optimization of medium composition for glycyrrhetinic acid monoglucuronide(GAMG) production using response surface methodology[J].Chinese Journal of Bioprocess Engineering,2009,7(05):29-33.
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响应面设计法优化单葡萄糖醛酸基甘草次酸(GAMG)发酵转化培养基
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
7
期数:
2009年05期
页码:
29-33
栏目:
出版日期:
2009-09-30

文章信息/Info

Title:
Optimization of medium composition for glycyrrhetinic acid monoglucuronide(GAMG) production using response surface methodology
文章编号:
1672-3678(2009)05-0029-05
作者:
李丽敏1邹树平2戴大章1李春12
1.北京理工大学 生命科学与技术学院,北京100081;
2.天津大学 化工学院,天津300072
Author(s):
LI Li-min1ZOU Shu-ping2DAI Da-zhang1LI Chun12
1. School of Life Science and Technology,Beijing Institute of Technology,Beijing 100081,China;
2. School of Chemical Engineering,Tianjin University,Tianjin 300072,China
关键词:
单葡萄糖醛酸基甘草次酸产紫青霉响应面设计
分类号:
TQ225
文献标志码:
A
摘要:
以甘草酸(glycyrrhizin,GL)为底物,利用产紫青霉(Penicillium purpurogenum Li3)液态发酵转化单葡萄糖醛酸甘草次酸(GAMG),采用响应面设计法对初始发酵培养基进行优化。用部分因子分析法研究原始发酵培养基各成分对响应值的显著程度,发现甘草酸(GL)、NaNO3和K2HPO4的质量浓度对发酵产生GAMG的影响显著(P<0.01)。用中心组合设计确立甘草酸、NaNO3和K2HPO4的适宜质量浓度分别为 2.8、3.0和0.8 g/L。在优化条件下进行发酵时,GAMG的转化率从75.49%提高到89.11%,比优化前提高了13.62%。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2008-12-19
基金项目:国家自然科学基金资助项目(20776017);北京市自然科学基金资助项目(5072028);北京理工大学基础研究基金资助项目(20060642004);北京理工大学研究生科技创新项目(GA200807)
作者简介:李丽敏(1986—),女,河北邯郸人,硕士研究生,研究方向:生物催化与酶工程;李春(联系人),教授,博士生导师,E-mail:lichun@bit.edu.cn.
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