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[1]孔静静,王长城,杨海麟,等.产HSA-CP融合蛋白毕赤酵母的发酵条件优化[J].生物加工过程,2009,7(05):25-28.
 KONG Jing-jing,WANG Chang-cheng,YANG Hai-lin,et al.Fermentation conditions of optimization of HSA-CP fusion protein expressed in Pichia pastoris[J].Chinese Journal of Bioprocess Engineering,2009,7(05):25-28.
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
7
期数:
2009年05期
页码:
25-28
栏目:
出版日期:
2009-09-30

文章信息/Info

Title:
Fermentation conditions of optimization of HSA-CP fusion protein expressed in Pichia pastoris
文章编号:
1672-3678(2009)05-0025-04
作者:
孔静静王长城杨海麟张玲周利苹金坚王武
江南大学 工业生物技术教育部重点实验室,无锡214122
Author(s):
KONG Jing-jingWANG Chang-chengYANG Hai-linZHANG LingZHOU Li-pingJIN JianWANG Wu
Key Laboratory of Industrial Biotechnology of the Ministry of Education,Jiangnan University,Wuxi 214122,China
关键词:
HSA-CP融合蛋白巴斯德毕赤酵母发酵优化
分类号:
Q814.4
文献标志码:
A
摘要:
为提高重组毕赤酵母生产人血清白蛋白C肽融合蛋白(HSA-CP)的产量和生产强度,在摇瓶条件下考察了甲醇诱导时间和浓度对目的蛋白产量的影响。结果表明,质量浓度10 g/L的甲醇诱导72 h最适于产物表达。通过对7 L发酵罐中各因素的优化,得到最佳条件为:初始甘油质量浓度10 g/L,30 ℃培养,菌体生长期和诱导期的pH及溶氧分别控制在pH 5.0、30%溶解O2或pH6.0、15%的溶解O2。10 g/L的甲醇诱导72 h,最终使干细胞质量浓度达到56.43 g/L,目的蛋白产量达368.45 mg/L。生产强度为3.920 mg/(L·h),目标蛋白的比生产速率为5.12 mg/(L·h)。

参考文献/References:

[1]Ido Y,Vindigni A,Chang K,et al.Prevention of vascular and neural dysfunction in diabetic rats by C-peptide[J].Science,1997,277:563-566.
[2]Johansson B L,Kernell A,Sjoberg S,et al.Influence of combined C-peptide and insulin administration on renal-function and metabolic control in diabetes type-1[J].Journal of Clinical Endocrinology and Metabolism,1993,77(4):976-981.
[3]Goodey A R.The production of heterologous plasma proteins [J].Trends Biotechnol,1993,11(10):430-433.
[4]周利苹,张莲芬,雷楗勇,等.人血清白蛋白C肽融合蛋白在毕赤酵母中的分泌表达[J].生物技术通报,2008(3):71-80.
Zhou Liping,Zhang Lianfen,Lei Jianyong,et al.Expression of the fusion protein HSA-CP in Pichia pastoris[J].Biotechnology Bulletin,2008(3):71-80.
[5]Kaoru Kobayashi,Shinobu Kuwae,Tomoshi Ohya,et al.High-level expression of recombinant human serum albumin from the methylotrophic yeast Pichia pastoris with minimal protease production and activation[J].Journal of Bioscience and Bioengineering,2000,89(1):55-61.
[6]Atef Ayed,Imen Rabhi,Koussay Dellagi,et al.High level production and purification of human interferon α2b in high cell density culture of Pichia pastoris[J].Enzyme and Microbial Technology,2008,42:173-180.
[7]杨如燕,李民,陈常庆,等.基因工程菌高密度发酵液中碳源物质甘油的定量检测及其浓度的优化控制[J].工业微生物,1999,29(1):25-28.
Yang Ruyan,Li Min,Chen Changqing,et al.Determination and optimization of glycerol during the high density fermentation of genetic engineered bacteria[J].Industrial Microorganism,1999,29(1):25-28.
[8]朱志钢,储炬,胡晓清,等.重组毕赤酵母发酵生产S-腺苷甲硫氨酸培养条件优化[J].工业微生物,2006,36(3):5-8.
Zhu Zhigang,Chu Ju,Hu Xiaoqing,et al.Optimization on culture conditions for producing S-adenosyl-L-methionine by recombinant Pichia pastoris[J].Industrial Microorganism,2006,36(3):5-8.


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备注/Memo

备注/Memo:
收稿日期:2008-11-28
基金项目:国家高新技术研究发展计划(863计划)资助项目(2006AA02Z153)
作者简介:孔静静(1984—),女,山东曲阜人,硕士研究生,研究方向:生物化学与分子生物学;王武(联系人),教授,博士生导师,E-mail:wangwu@jiangnan.edu.cn
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